PXD043134

PXD043134 is an original dataset announced via ProteomeXchange.

Title	ZBTB48 is a pioneer factor regulating B-cell-specific CIITA expression
Description	CIITA is the master regulator of MHC II genes expression and hence the adaptive immune response. CIITA expression itself is tightly regulated by three cell type-specific promoters, pI, pIII, and by pIV, and can also be induced by IFNg in non-immune cells. While key regulatory elements have been identified within these promoters, knowledge of transcription factors regulating CIITA is incomplete. Here, we demonstrate that the telomere-binding protein and transcriptional activator ZBTB48 directly binds to both critical activating elements within CIITA pIII and is essential for its gene expression. ZBTB48 establishes open chromatin at CIITA pIII upstream of activating H3K4me3 modifications both priming CIITA transcription for IFNg-induction and ensuring constitutive expression in primary murine B cells. Hence, ZBTB48 acts as a molecular on-off-switch for B-cell-specific CIITA expression.
HostingRepository	PRIDE
AnnounceDate	2024-12-22
AnnouncementXML	Submission_2024-12-21_23:58:57.312.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dennis Kappei
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-20 03:10:15	ID requested
⏵ 1	2024-12-21 23:58:58	announced

10.1038/s44318-024-00306-y;

Rane G, Kuan VLS, Wang S, Mok MMH, Khanchandani V, Hansen J, Norvaisaite I, Zulkaflee N, Yong WK, Jahn A, Mukundan VT, Shi Y, Osato M, Li F, Kappei D, ZBTB48 is a priming factor regulating B-cell-specific CIITA expression. EMBO J, 43(24):6236-6263(2024) [pubmed]

submitter keyword: MHC II, adaptive immunity, B cell, zinc finger, pioneer factor, molecular switch, CIITA, ZBTB48

Dennis Kappei
contact affiliation	Cancer Science Institute of Singapore (CSI), National University of Singapore (NUS)
contact email	dennis.kappei@nus.edu.sg
lab head
Dennis Kappei
contact affiliation	Cancer Science Institute of Singapore
contact email	dennis.kappei@nus.edu.sg
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/12/PXD043134

PRIDE project URI

• PRIDE
  1. PXD043134
     1. Label: PRIDE project
     2. Name: ZBTB48 is a pioneer factor regulating B-cell-specific CIITA expression