PXD043100

PXD043100 is an original dataset announced via ProteomeXchange.

Title	CCAR-1 regulates the alternative splicing of multiple genes in Caenorhabditis elegans
Description	The Cell Division Cycle and Apoptosis Regulator (CCAR) protein family members have recently emerged as regulators of alternative splicing and transcription, as well as having other key physiological functions. For example, mammalian CCAR2/DBC1 forms a complex with the zinc factor protein ZNF326 to integrate alternative splicing with RNA polymerase II transcriptional elongation in AT-rich regions of the DNA. Additionally, Caenorhabditis elegans CCAR-1, a homolog to mammalian CCAR2, facilitates the alternative splicing of the perlecan unc-52 gene. However, much about the CCAR family's role in alternative splicing is unknown. We are interested in uncovering the role of the CCAR family in alternative splicing in vivo using Caenorhabditis elegans. We examined the role of CCAR-1 in genome-wide alternative splicing and identified new alternative splicing targets of CCAR-1 using RNA sequencing. Also, we found that CCAR-1 interacts with the spliceosome factors UAF-1 and UAF-2 using mass spectrometry, and that knockdown of ccar-1 affects alternative splicing patterns, motility, and proteostasis of UAF-1 mutant worms. Collectively, we demonstrate a role for CCAR-1 in the regulation of global alternative splicing in C. elegans and in conjunction with UAF-1
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:31:39.984.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dale Chaput
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-19 06:44:01	ID requested
1	2024-02-26 08:05:05	announced
⏵ 2	2024-10-22 06:31:40	announced	2024-10-22: Updated project metadata.

10.1080/15476286.2023.2289707;

Lugano DI, Barrett LN, Chaput D, Park MA, Westerheide SD, CCAR-1 works together with the U2AF large subunit UAF-1 to regulate alternative splicing. RNA Biol, 21(1):1-11(2024) [pubmed]

submitter keyword: C. elegans, alternative splicing, CCAR-1

Sandy Westerheide
contact affiliation	Associate Professor, Molecular Biosciences, College of Arts and Science, University of South Florida
contact email	swesterheide@usf.edu
lab head
Dale Chaput
contact affiliation	University of South Florida
contact email	chaput@usf.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD043100

PRIDE project URI

• PRIDE
  1. PXD043100
     1. Label: PRIDE project
     2. Name: CCAR-1 regulates the alternative splicing of multiple genes in Caenorhabditis elegans