PXD043093

PXD043093 is an original dataset announced via ProteomeXchange.

Title	UV-inducible crosslinking IP-MS to elucidate the role of nuclear Hsp104 during quiescence in Saccharomyces cerevisiae
Description	In this study, we utilize high-content microscopy screening and quantitative proteomics to establish a function of nuclear Hsp104 during aging. We find that the cytosolic-nuclear partitioning of Hsp104 is critical to maintain nuclear proteostasis in quiescent cells. Whereas high global translation rates in rapidly growing cells constrain Hsp104 to the cytosol, a decrease in protein biosynthesis re-directs the disaggregase to the nucleus dependent on a specific C-terminal motif. Nuclear Hsp104 interacts with latent eIF2 translation initiation subcomplexes and suppresses protein aggregation. This protects the dormant translation machinery from age-induced damage, enabling the rapid resumption of protein synthesis upon re-entry into the cell cycle. In this project two separate IP-MS experiments are included, KGSBVK (IP-MS, 9 samples) and GMCAVK (UV-inducible crosslinking IP-MS, 12 samples).
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:21:47.325.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Georgios Mermelekas
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-19 03:54:59	ID requested
1	2024-01-12 05:05:15	announced
⏵ 2	2024-10-22 06:21:47	announced	2024-10-22: Updated project metadata.

10.1038/S41467-023-44538-8;

submitter keyword: p-benzoyl-L-phenylalanine,Yeast, quiescence, UV-inducible crosslinking, Nuclear protein quality control, IP-MS, Ageing, Co-immunoprecipitation, LC-MS/MS, Translation, Hsp104, Proteomics, Disaggregation

Janne Lehtiö
contact affiliation	Department of Oncology, Karolinska Inasitutet, Scilifelab, Stockholm, Sweden
contact email	janne.lehtio@ki.se
lab head
Georgios Mermelekas
contact affiliation	Karolinska Institutet
contact email	georgios.mermelekas@scilifelab.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD043093

PRIDE project URI

• PRIDE
  1. PXD043093
     1. Label: PRIDE project
     2. Name: UV-inducible crosslinking IP-MS to elucidate the role of nuclear Hsp104 during quiescence in Saccharomyces cerevisiae