PXD043086

PXD043086 is an original dataset announced via ProteomeXchange.

Title	An integrative proteomics approach to identify novel therapeutic targets for SPINK1-positive prostate cancer.
Description	Dysregulated phosphorylation of proteins perturbs the activity of several biological pathways leading to tumorigenesis and metastases. Integrated proteomics studies offer an in-depth comprehension of cancer pathobiology, and could untangle its complex circuitries. To decipher the signaling involved in SPINK1-mediated tumorigenesis, we performed label-free quantitative proteome and phosphoproteome profiling of small hairpin RNA -mediated SPINK1 silenced (shSPINK1) and control small hairpin RNA Scrambled (shSCRM) 22RV1 cells. Our integrated proteomics data revealed ~4000 proteins and enriched ~6000 phosphopeptides in each biological replicate. Of these, 367 proteins and 807 phosphopeptides were found to be significantly altered between shSCRM and shSPINK1 cells.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:31:40.125.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bushra Ateeq
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-19 00:55:04	ID requested
1	2024-02-26 09:11:53	announced
⏵ 2	2024-10-22 06:31:40	announced	2024-10-22: Updated project metadata.

10.1016/j.isci.2024.108794;

Manzar N, Khan UK, Goel A, Carskadon S, Gupta N, Palanisamy N, Ateeq B, An integrative proteomics approach identifies tyrosine kinase KIT as a therapeutic target for SPINK1-positive prostate cancer. iScience, 27(3):108794(2024) [pubmed]

submitter keyword: Integrative proteome profiling,Prostate Cancer, SPINK1

Dr. Bushra Ateeq
contact affiliation	Professor & Joy-Gill Chair Department of Biological Sciences and Bioengineering Indian Institute of Technology Kanpur 208016 Uttar Pradesh India
contact email	bushra@iitk.ac.in
lab head
Bushra Ateeq
contact affiliation	Indian Institute of Technology Kanpur
contact email	bushra@iitk.ac.in
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD043086

PRIDE project URI

• PRIDE
  1. PXD043086
     1. Label: PRIDE project
     2. Name: An integrative proteomics approach to identify novel therapeutic targets for SPINK1-positive prostate cancer.