PXD043080

PXD043080 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomic analysis of seed germination between allotetraploid cottons Gossypium hirsutum and Gossypium barbadense
Description	Seed germination, a key initial event in the plant life cycle, directly affects the yield and quality of cotton. Gossypium barbadense and Gossypium hirsutum are gradually evolved through polyploidization resulting in different characteristics, and the existence of this interspecific variation lacks genetic and molecular explanation. In this study, we compared the proteomes between G. barbadense and G. hirsutum during seed germination. In all we identified 2741 proteins for G. barbadense and 3759 proteins for G. hirsutum. Through functional enrichment, we found that seeds germinate by first repairing damaged DNA and proteins, as well as mobilizing stored substances such as carbohydrates, proteins and lipids for energy production to initiate life activities, followed by progressive development through cell division and other processes. We also performed homology analysis and found 21711 orthogroups including 2761 species-specific orthogroups for G. hirsutum and 527 ones for G. barbadense. Further differential analysis in 915 single-copy orthogroups between the two cottons was conducted, indicating that most of the differentially expressed proteins in G. barbadense were related to carbon metabolism, while most of the differentially expressed proteins in G. hirsutum were associated with stress response.
HostingRepository	iProX
AnnounceDate	2023-06-19
AnnouncementXML	Submission_2024-05-29_21:01:04.939.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aishuake Huwanixi
SpeciesList	scientific name: Gossypium hirsutum; NCBI TaxID: 3635; scientific name: Gossypium barbadense; NCBI TaxID: 3634;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-18 19:47:05	ID requested
1	2023-06-18 19:47:21	announced
⏵ 2	2024-05-29 21:01:05	announced	2024-05-30: Update information.

Huwanixi A, Peng Z, Li S, Zhou Y, Zhao S, Wan C, Comparative proteomic analysis of seed germination between allotetraploid cotton Gossypium hirsutum and Gossypium barbadense. J Proteomics, 297():105130(2024) [pubmed]

submitter keyword: proteome, Gossypium, LC-MS/MS, seed germination, allopolyploid

Cuihong Wan
contact affiliation	Central China Normal University
contact email	ch_wan@ccnu.edu.cn
lab head
Aishuake Huwanixi
contact affiliation	Central China Normal University
contact email	ishuak1999@163.com
dataset submitter

iProX dataset URI