PXD043056

PXD043056 is an original dataset announced via ProteomeXchange.

Title	Siderophore specifities of ChtA and ActA, two TonB-dependent transporters of Pseudomonas
Description	Iron is an essential nutrient for the opportunistic pathogen Pseudomonas aeruginosa, as for almost all living organisms. To access this element, the pathogen is able to express at least 15 different iron-uptake pathways, the vast majority involving small iron chelators called siderophores. Indeed, P. aeruginosa produces two siderophores, pyoverdine and pyochelin, but can also use many produced by other microorganisms. This implies that the bacterium expresses appropriate TonB-dependent transporters (TBDTs) at the outer membrane to import the ferric form of each of the siderophores used. These transporters are highly selective for a given ferri-siderophore complex or for siderophores with similar chemical structures. Here, we show that P. aeruginosa can also use rhizoferrin, staphyloferrin A, aerobactin, and schizokinen as siderophores to access iron. Growth assays in iron-restricted conditions and 55Fe uptake assays showed that the two alpha-carboxylate type siderophores rhizoferrin-Fe and staphyloferrin A-Fe are transported into P. aeruginosa cells by the TBDT ActA (PA3268). Among the mixed alpha-carboxylate/hydroxamate type siderophores, we found aerobactin-Fe to be transported by ChtA (as previously described) and schizokinen-Fe by ChtA and another unidentified TBDT.
HostingRepository	PRIDE
AnnounceDate	2023-09-21
AnnouncementXML	Submission_2023-09-21_10:09:52.773.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD043056
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	LaurianeKuhn
SpeciesList	scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-16 06:23:34	ID requested
⏵ 1	2023-09-21 10:09:53	announced

10.6019/PXD043056;

submitter keyword: Pseudomonas, ChtA, ActA,siderophore

IsabelleSchalk
contact affiliation	CNRS, University of Strasbourg, UMR7242, UMR7242, ESBS, Bld Sébastien Brant, F-67412 Illkirch, Strasbourg, France
contact email	isabelle.schalk@unistra.fr
lab head
LaurianeKuhn
contact affiliation	CNRS, IBMC, FRC1589
contact email	l.kuhn@ibmc-cnrs.unistra.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD043056

PRIDE project URI

• PRIDE
  1. PXD043056
     1. Label: PRIDE project
     2. Name: Siderophore specifities of ChtA and ActA, two TonB-dependent transporters of Pseudomonas