PXD043051

PXD043051 is an original dataset announced via ProteomeXchange.

Title	Horizontal acquisition of a DNA ligase improves DNA damage tolerance in eukaryotes
Description	Bdelloid rotifers are part of the restricted circle of multicellular animals that can withstand a wide range of genotoxic stresses at any stage of their life cycle. In this study, bdelloid rotifer Adineta vaga was used as a model to decipher the molecular basis of their extreme tolerance. Proteomic analysis showed that a specific DNA ligase, different from those usually involved in DNA repair in eukaryotes, is strongly over-represented upon ionizing radiation. A phylogenetic analysis revealed its orthology to prokaryotic DNA ligase E, and its horizontal acquisition by bdelloid rotifers and plausibly other eukaryotes. The fungus Mortierella verticillata, having a single copy of this DNA Ligase E homolog, also exhibits an increased radiation tolerance with an over-expression of this DNA ligase E following X-ray exposure. We also provide evidence that A. vaga ligase E is a major contributor of DNA breaks ligation activity, which is a common step of all important DNA repair pathways. Consistently, its heterologous expression in human cell lines significantly improved their radio-tolerance. Overall, this study highlights the potential of horizontal gene transfers in eukaryotes, and their contribution to the adaptation to extreme conditions.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:24:53.660.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD043051
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Marc Dieu
SpeciesList	scientific name: Adineta vaga; NCBI TaxID: 104782;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-16 03:07:12	ID requested
1	2024-01-24 10:13:13	announced
⏵ 2	2024-10-22 06:24:54	announced	2024-10-22: Updated project metadata.

10.1038/S41467-023-43075-8;

10.6019/PXD043051;

submitter keyword: LC-MSMS, DNA repair, X-ray,Bdelloid rotifers

Karine Van
contact affiliation	Université Libre de Bruxelles, Molecular Biology and Evolution, Brussels, 1050, Belgium Université de Namur, Laboratory of Evolutionary Genetics and Ecology, Namur, 5000, Belgium
contact email	karine.van.doninck@ulb.be
lab head
Marc Dieu
contact affiliation	University of Namur - MaSUN
contact email	marc.dieu@unamur.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD043051

PRIDE project URI

• PRIDE
  1. PXD043051
     1. Label: PRIDE project
     2. Name: Horizontal acquisition of a DNA ligase improves DNA damage tolerance in eukaryotes