PXD043050

PXD043050 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of fresh and liquid stored turkey spermatozoa
Description	The liquid storage of turkey semen without the loss of fertilizing ability is of practical interest to poultry industry. However, fertility rates from liquid stored turkey semen decline within a few hours. A clear cause of the decline in spermatozoa quality is unknown. Therefore, the purpose of the present study was to monitor dynamic of proteomic changes of spermatozoa during liquid storage at three time points (2, 24 and 48h) by 2-dimensional difference in-gel electrophoresis (2D-DIGE) coupled with a matrix-assisted laser desorption/ionization mass (MALDI TOF/TOF) spectrometry. A total of 57 protein spots were differentially expressed in fresh and stored spermatozoa; 42 spots were more- and 15 were less- abundant in spermatozoa after 48 h of semen storage. Most of the proteins that changed in response to liquid storage were related to i) flagellum dependent cell motility, ii) energy derivation by oxidation of organic compounds and iii) introduction of fertilization, suggesting complexity of the processes leading to decreases of stored semen quality.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_09:24:53.717.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mariola Słowińska
SpeciesList	scientific name: Meleagris gallopavo (Common turkey); NCBI TaxID: 9103;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	autoflex

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-16 03:06:44	ID requested
⏵ 1	2024-01-26 09:24:54	announced

10.1016/j.theriogenology.2023.12.026;

Pardyak L, Liszewska E, Judycka S, Machci, ń, ska-Zieli, ń, ska S, Karol H, Dietrich MA, Goj, ł, o E, Arent Z, Bili, ń, ska B, Rusco G, Iaffaldano N, Ciereszko A, S, ł, owi, ń, ska M, Liquid semen storage-induced alteration in the protein composition of turkey (Meleagris gallopavo) spermatozoa. Theriogenology, 216():69-81(2024) [pubmed]

submitter keyword: turkey, MALDI TOF/TOF, 2D-DIGE, spermatozoa

Mariola Słowińska
contact affiliation	Institute of Animal Reproduction and Food Research, Polish Academy of Sciences in Olsztyn
contact email	m.slowinska@pan.olsztyn.pl
lab head
Mariola Słowińska
contact affiliation	Institute of Animal Reproduction and Food Research of Polish Academy of Sciences
contact email	m.slowinska@pan.olsztyn.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD043050

PRIDE project URI

• PRIDE
  1. PXD043050
     1. Label: PRIDE project
     2. Name: Proteomic analysis of fresh and liquid stored turkey spermatozoa