PXD043048

PXD043048 is an original dataset announced via ProteomeXchange.

Title	STXBP5 prevents reannealing of autophagic cis-SNARE disassembled by NSF/αSNAP
Description	We generated a HEK293 cell line expressing ZZ-TEV-FLAG-bait protein by Lipofectamine 3000 transfection. The expression level of bait protein is controlled to endogenous level by addition of 2 ng/mL doxycycline. Note the combined treatment of Torin1 and CQ was applied for STX17. The tandem affinity purification of tagged bait protein was performed in a physiology buffer condition [20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.5% Igepal CA-630, 1 mM EDTA, protease inhibitor cocktail (Roche)]. The eluted protein mixtures were subjected to SDS/PAGE and further analyzed by mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2023-09-18
AnnouncementXML	Submission_2023-09-17_20:01:48.819.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	LinsenLi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-16 01:05:46	ID requested
⏵ 1	2023-09-17 20:01:49	announced

Dataset with its publication pending

submitter keyword: STXBP5, alphaSNAP,autophagy, disassemble, SNARE, NSF

QingZhong
contact affiliation	Shanghai jiaotong University School of Medicine
contact email	qingzhong@shsmu.edu.cn
lab head
LinsenLi
contact affiliation	Shanghai Jiaotong University
contact email	linsenli@shsmu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD043048

PRIDE project URI

• PRIDE
  1. PXD043048
     1. Label: PRIDE project
     2. Name: STXBP5 prevents reannealing of autophagic cis-SNARE disassembled by NSF/αSNAP