PXD043030

PXD043030 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Xenopus laevis sperm extract IEX and SEC Fractionation
Description	Sperm were flushed from freshly dissected Xenopus laevis (J strain) testis using Marks Modified Ringers solution, and collected by centrifugation. Purified sperm were lysed in buffer containing 1% n-Dodecy-beta-D-Maltoside and the clarified extract was fractionated on either a mixed bed ion exchange column or an SEC. Column fractions were reduced, alkylated, and digested with trypsin for mass spectrometry as follows. IEX samples were run on an Orbitrap Fusion using a 75 minute topspeed CID method, while SEC samples were run on an Orbitrap Fusion Lumos using a 75 minute topspeed HCD method. Data analysis was using the MSBlender pipeline, and an Xenla database collapsed at the eukaryote level using eggNOG mapper.
HostingRepository	MassIVE
AnnounceDate	2025-07-29
AnnouncementXML	Submission_2025-07-29_12:16:55.508.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ophelia Papoulas
SpeciesList	scientific name: Xenopus laevis; common name: African clawed frog; NCBI TaxID: 8355;
ModificationList	Carbamidomethyl
Instrument	Orbitrap Fusion Lumos; Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-15 12:34:27	ID requested
⏵ 1	2025-07-29 12:16:55	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Xenopus laevis, sperm, Ion Exchange, SEC, LECA

submitter keyword: Xenopus laevis, sperm, Ion Exchange, SEC, LECA

Contact List

Edward Marcotte
contact affiliation	University of Texas-Austin
contact email	marcotte@icmb.utexas.edu
lab head
Ophelia Papoulas
contact affiliation	University of Texas at Austin
contact email	papoulas@austin.utexas.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v05/MSV000092180/