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PXD043010

PXD043010 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDiscovery, Verification, and Validation of Walnut Protein Marker Peptides Using LC-MS/MS Approaches
DescriptionA key requirement of liquid chromatography-mass spectrometry (LC-MS)-based allergenic food protein analysis methods is to use protein marker peptides with good analytical performances in LC-MS analysis of commercial processed foods. In this study, we developed a multistage walnut protein marker peptide selection strategy involving marker peptide discovery and verification and LC-MS validation of chemically equivalent stable isotope-labeled peptides. This strategy proposed three walnut protein marker peptides, including two new marker peptides. Our LC-MS-based walnut protein analysis method using the three stable isotope-labeled peptides showed acceptable linearity (R2 >0.99), matrix effects (coefficient of variation <±15%), sensitivity (limit of detection >0.3 pg/μL, limit of quantification >0.8 pg/μL), recovery (85.1–103.4%), accuracy, and precision (coefficient of variation <10%). In conclusion, our multistage marker peptide selection strategy effectively selects specific protein marker peptides for sensitive detection and absolute quantification of walnut proteins in LC-MS analysis of commercial processed foods.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:59:13.305.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKyungdo Kim
SpeciesList scientific name: Juglans regia (English walnut); NCBI TaxID: 51240;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentTSQ Altis; Q Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-06-15 03:39:41ID requested
12023-07-31 03:44:40announced
22023-11-14 06:49:27announced2023-11-14: Updated project metadata.
32024-10-22 05:59:13announced2024-10-22: Updated project metadata.
Publication List
10.1016/J.FOODCHEM.2023.136889;
Keyword List
submitter keyword: Food allergy, LC-MS, Validation,Walnut marker peptide
Contact List
Zee-yong Park
contact affiliationSchool of Life Science, Gwangju Institute of Science and Technology, Cheomdangwagiro123, Buk-gu, Gwangju, 61005, Republic of Korea
contact emailzeeyong@gist.ac.kr
lab head
Kyungdo Kim
contact affiliationGwnagju Institute of Science and Technology
contact emailrlarudeh10@gm.gist.ac.kr
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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