PXD043010

PXD043010 is an original dataset announced via ProteomeXchange.

Title	Discovery, Verification, and Validation of Walnut Protein Marker Peptides Using LC-MS/MS Approaches
Description	A key requirement of liquid chromatography-mass spectrometry (LC-MS)-based allergenic food protein analysis methods is to use protein marker peptides with good analytical performances in LC-MS analysis of commercial processed foods. In this study, we developed a multistage walnut protein marker peptide selection strategy involving marker peptide discovery and verification and LC-MS validation of chemically equivalent stable isotope-labeled peptides. This strategy proposed three walnut protein marker peptides, including two new marker peptides. Our LC-MS-based walnut protein analysis method using the three stable isotope-labeled peptides showed acceptable linearity (R2 >0.99), matrix effects (coefficient of variation <±15%), sensitivity (limit of detection >0.3 pg/μL, limit of quantification >0.8 pg/μL), recovery (85.1–103.4%), accuracy, and precision (coefficient of variation <10%). In conclusion, our multistage marker peptide selection strategy effectively selects specific protein marker peptides for sensitive detection and absolute quantification of walnut proteins in LC-MS analysis of commercial processed foods.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:59:13.305.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kyungdo Kim
SpeciesList	scientific name: Juglans regia (English walnut); NCBI TaxID: 51240;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TSQ Altis; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-15 03:39:41	ID requested
1	2023-07-31 03:44:40	announced
2	2023-11-14 06:49:27	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:59:13	announced	2024-10-22: Updated project metadata.

10.1016/J.FOODCHEM.2023.136889;

submitter keyword: Food allergy, LC-MS, Validation,Walnut marker peptide

Zee-yong Park
contact affiliation	School of Life Science, Gwangju Institute of Science and Technology, Cheomdangwagiro123, Buk-gu, Gwangju, 61005, Republic of Korea
contact email	zeeyong@gist.ac.kr
lab head
Kyungdo Kim
contact affiliation	Gwnagju Institute of Science and Technology
contact email	rlarudeh10@gm.gist.ac.kr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD043010

PRIDE project URI

• PRIDE
  1. PXD043010
     1. Label: PRIDE project
     2. Name: Discovery, Verification, and Validation of Walnut Protein Marker Peptides Using LC-MS/MS Approaches