PXD043010 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Discovery, Verification, and Validation of Walnut Protein Marker Peptides Using LC-MS/MS Approaches |
Description | A key requirement of liquid chromatography-mass spectrometry (LC-MS)-based allergenic food protein analysis methods is to use protein marker peptides with good analytical performances in LC-MS analysis of commercial processed foods. In this study, we developed a multistage walnut protein marker peptide selection strategy involving marker peptide discovery and verification and LC-MS validation of chemically equivalent stable isotope-labeled peptides. This strategy proposed three walnut protein marker peptides, including two new marker peptides. Our LC-MS-based walnut protein analysis method using the three stable isotope-labeled peptides showed acceptable linearity (R2 >0.99), matrix effects (coefficient of variation <±15%), sensitivity (limit of detection >0.3 pg/μL, limit of quantification >0.8 pg/μL), recovery (85.1–103.4%), accuracy, and precision (coefficient of variation <10%). In conclusion, our multistage marker peptide selection strategy effectively selects specific protein marker peptides for sensitive detection and absolute quantification of walnut proteins in LC-MS analysis of commercial processed foods. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:59:13.305.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Kyungdo Kim |
SpeciesList | scientific name: Juglans regia (English walnut); NCBI TaxID: 51240; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | TSQ Altis; Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-06-15 03:39:41 | ID requested | |
1 | 2023-07-31 03:44:40 | announced | |
2 | 2023-11-14 06:49:27 | announced | 2023-11-14: Updated project metadata. |
⏵ 3 | 2024-10-22 05:59:13 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: Food allergy, LC-MS, Validation,Walnut marker peptide |
Contact List
Zee-yong Park |
contact affiliation | School of Life Science, Gwangju Institute of Science and Technology, Cheomdangwagiro123, Buk-gu, Gwangju, 61005, Republic of Korea |
contact email | zeeyong@gist.ac.kr |
lab head | |
Kyungdo Kim |
contact affiliation | Gwnagju Institute of Science and Technology |
contact email | rlarudeh10@gm.gist.ac.kr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD043010
- Label: PRIDE project
- Name: Discovery, Verification, and Validation of Walnut Protein Marker Peptides Using LC-MS/MS Approaches