PXD042987

PXD042987 is an original dataset announced via ProteomeXchange.

Title	Nuclear Hsp104 safeguards the dormant translation machinery during quiescence
Description	The resilience of cellular proteostasis declines with age, which drives protein aggregation and compromises viability. The nucleus has emerged as a key quality control compartment that unloads the cytosolic protein biosynthesis system from misfolded proteins. Here, we find that age-associated metabolic cues target the yeast protein disaggregase Hsp104 to the nucleus to maintain a functional nuclear proteome during quiescence. The switch to respiratory metabolism and the accompanying decrease in translation rates direct cytosolic Hsp104 to the nucleus to interact with latent translation initiation factor eIF2 subcomplexes and to suppress protein aggregation. Hindering Hsp104 from entering the nucleus in quiescent cells results in delayed re-entry into the cell cycle due to compromised resumption of protein synthesis. In sum, we report that cytosolic-nuclear partitioning of the Hsp104 disaggregase is a critical mechanism to protect the latent protein synthesis machinery during quiescence, ensuring the rapid restart of translation once nutrients are replenished.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:21:45.571.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ignasi Forne
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-14 09:30:38	ID requested
1	2024-01-11 23:57:03	announced
⏵ 2	2024-10-22 06:21:45	announced	2024-10-22: Updated project metadata.

10.1038/S41467-023-44538-8;

submitter keyword: Quiescence, eIF2, Aging, Nuclear protein quality control, Protein aggregation,Disaggregation, Translation, Proteasome, Hsp104, Ribosome reactivation, BioID

Sabrina Büttner
contact affiliation	Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, 10691 Stockholm, Sweden
contact email	sabrina.buettner@su.se
lab head
Ignasi Forne
contact affiliation	Biomedical Center-LMU
contact email	ignasi.forne@lrz.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD042987

PRIDE project URI

• PRIDE
  1. PXD042987
     1. Label: PRIDE project
     2. Name: Nuclear Hsp104 safeguards the dormant translation machinery during quiescence