PXD042983

PXD042983 is an original dataset announced via ProteomeXchange.

Title	BLM helicase protein negatively regulates stress granule formation through unwinding RNA G-quadruplex structures
Description	We ran native 5% polyacrylamide-TBE gel for electro mobility shift assay (EMSA) for DNA/RNA G4 structures with or without recombinant human core-BLM (cBLM) protein, which was extracted and purified from E.coli in order to show binding of the RNA/DNA by this protein. To further evidence, we cut the equivalent gel area where the shifted band is located for all the wells (DNA only, DNA+cBLM, RNA, RNA+cBLM and cBLM only) and anlayzed by LC-MS/MS to identify human BLM and to see if there are some E.coli contaminations in those specific bands. Moreover, we analyzed the stock solution of the recombinant cBLM as general control.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:11:33.740.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yehuda Danino
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-14 07:28:10	ID requested
1	2023-10-24 13:12:44	announced
⏵ 2	2023-11-14 09:11:34	announced	2023-11-14: Updated project metadata.

Danino YM, Molitor L, Rosenbaum-Cohen T, Kaiser S, Cohen Y, Porat Z, Marmor-Kollet H, Katina C, Savidor A, Rotkopf R, Ben-Isaac E, Golani O, Levin Y, Monchaud D, Hickson ID, Hornstein E, BLM helicase protein negatively regulates stress granule formation through unwinding RNA G-quadruplex structures. Nucleic Acids Res, 51(17):9369-9384(2023) [pubmed]

submitter keyword: BLM, SG, EMSA, G-quadruplex, LC-MS/MS

Eran Hornstein
contact affiliation	The Weizmann Institute of Science
contact email	era.hornstein@weizmann.ac.il
lab head
Yehuda Danino
contact affiliation	Weizmann Institute of Science
contact email	yehuda015@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD042983

PRIDE project URI

• PRIDE
  1. PXD042983
     1. Label: PRIDE project
     2. Name: BLM helicase protein negatively regulates stress granule formation through unwinding RNA G-quadruplex structures