PXD042969

PXD042969 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Limited proteolysis for PTM analysis
Description	PTMs on a mycobacterial DNA-binding protein with a long, lysine-rich, intrinsically disorders region were analyzed by limited proteolysis with trypsin, unveiling a massive methylation of most lysine residues of the protein in quantitative manner, which was lacked in the recombinant version of the protein expressed in E. coli.
HostingRepository	jPOST
AnnounceDate	2024-06-14
AnnouncementXML	Submission_2024-06-13_08:00:27.676.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yutaka Yoshida
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Mycolicibacterium smegmatis MC2 155; NCBI TaxID: 246196; scientific name: Mycobacterium tuberculosis; NCBI TaxID: 1773;
ModificationList	S-carboxamidomethyl-L-cysteine; N6-acetyl-L-lysine; 2-pyrrolidone-5-carboxylic acid (Gln); L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; N6-methyl-L-lysine; N6,N6-dimethyl-L-lysine; N6,N6,N6-trimethyl-L-lysine (from L-lysinium residue); 4x(1)H,4x(12)C-labeled N6-succinyl-L-lysine; deamidated L-asparagine; deamidated L-glutamine
Instrument	TripleTOF 5600

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-13 22:35:36	ID requested
⏵ 1	2024-06-13 08:00:28	announced

Publication List

Yoshida Y, Nishiyama A, Suameitria Dewi DNS, Yamazaki T, Yokoyama A, Kobayashi D, Kondo H, Ozeki Y, Matsumoto S, Limited proteolysis of mycobacterial DNA-binding protein 1 with an extended, lysine-rich, intrinsically disordered region to unveil posttranslational modifications. Biochem Biophys Res Commun, 681():111-119(2023) [pubmed]

Yoshida Y, Nishiyama A, Suameitria Dewi DNS, Yamazaki T, Yokoyama A, Kobayashi D, Kondo H, Ozeki Y, Matsumoto S, Limited proteolysis of mycobacterial DNA-binding protein 1 with an extended, lysine-rich, intrinsically disordered region to unveil posttranslational modifications. Biochem Biophys Res Commun, 681():111-119(2023) [pubmed]

Keyword List

submitter keyword: limited proteolysis, trypsin, intrinsically disordered region, PTMs on lysine, protein methylation

submitter keyword: limited proteolysis, trypsin, intrinsically disordered region, PTMs on lysine, protein methylation

Contact List

Sohkichi Matsumoto
lab head
Yutaka Yoshida
contact affiliation	Niigata University Graduate School of Medical and Dental Sciences
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002198/