PXD042967

PXD042967 is an original dataset announced via ProteomeXchange.

Title	Multifaceted proteome analysis at solubility, redox, and expression dimensions for target identification
Description	Multifaceted interrogation of the proteome deepens the system-wide understanding of biological systems; however, mapping the redox changes in the proteome has so far been significantly less informative than expression and solubility/stability analyses. Here, we devise the first high-throughput redox proteomics approach integrated with expression analysis (REX) and combine it with Proteome Integral Solubility Alteration (PISA) assay. The whole PISA-REX experiment in three biological replicates is multiplexed in a single tandem mass tag set (TMTpro). For benchmarking this compact PISA-REX tool, we analyzed HCT116 cells treated with auranofin. Then we applied PISA-REX to study the targets of Pladienolide B in SW620 cells, proteome remodeling upon stimulation of human monocytes by interferon α, and to paired primary and metastatic colon cancer cells. Providing comprehensive multifaceted information on the proteome, the compact PISA-REX has the potential to become an industry-standard in proteomics and to open new windows into the biology of health and disease.
HostingRepository	PRIDE
AnnounceDate	2024-08-10
AnnouncementXML	Submission_2024-08-10_03:17:10.990.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Amir Ata Saei
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF; Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-13 22:23:07	ID requested
⏵ 1	2024-08-10 03:17:11	announced

10.1002/ADVS.202401502;

submitter keyword: Multidimensional, proteomics, reactive oxygen species, mass spectrometry, interferon, stability

Roman A. Zubarev
contact affiliation	Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden
contact email	roman.zubarev@ki.se
lab head
Amir Ata Saei
contact affiliation	Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
contact email	amirata.saei.dibavar@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD042967

PRIDE project URI

• PRIDE
  1. PXD042967
     1. Label: PRIDE project
     2. Name: Multifaceted proteome analysis at solubility, redox, and expression dimensions for target identification