PXD042962

PXD042962 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiling and tumor microenvironment characterization reveal molecular and immunological hallmarks of left-sided and right-sided colon cancer tumorigenesis
Description	The protein landscapes of LSCC and RSCC were constructed, and the hallmarks of tumorigenesis and characteristics of TME for LSCC and RSCC were identified. These findings improved our understanding of LSCC and RSCC and may facilitate the development of diagnostic or therapeutic targets for LSCC and RSCC. Collected samples were digested as previously described. Briefly, adding appropriate volume of lysate containing 1% protease inhibitor cocktail into the samples. Then, the samples were further ultrasonically lysed (2s-on, 3s-off) for 10 min until the solution became transparent. After centrifugation, supernatant was absorbed into a new EP tube to obtain protein extract. Total protein was quantified by BCA method. After quantification, DTT was added to the sample to make the final concentration of the solution was 5 mM. The EP tube was placed at 56 ℃ for 60 min. After the solution was cooled to room temperature, 55 mM IAM was added to block free sulfhydryl at room temperature for 45 min in the dark. Next, 60 μg
HostingRepository	iProX
AnnounceDate	2023-06-13
AnnouncementXML	Submission_2024-01-03_20:04:26.055.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hongzhen Tang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-13 19:13:48	ID requested
⏵ 1	2024-01-03 20:04:26	announced

Hong Z, Wang T, Wang W, Jing H, Tang H, Xu M, Pan C, Mu X, Zhang D, Gao G, Gao Z, Luo H, Zhou Y, Proteomic Profiling and Tumor Microenvironment Characterization Reveal Molecular and Immunological Hallmarks of Left-Sided and Right-Sided Colon Cancer Tumorigenesis. J Proteome Res, 22(9):2973-2984(2023) [pubmed]

submitter keyword: LSCC, RSCC, DIA-MS, proteomic profile, tumorigenesis, TME

Yi Zhou
contact affiliation	Department of Colorectal Surgery, Tianjin Union Medical Center; Tianjin Institute of Coloproctology, Tianjin, China
contact email	jj_158@126.com
lab head
Hongzhen Tang
contact affiliation	Shenzhen Engineering Center for Translational Medicine of Precision Cancer Immunodiagnosis and Therapy, YuceBio Technology Co., Ltd, Shenzhen, China
contact email	tanghongzhen34@gmail.com
dataset submitter

iProX dataset URI