PXD042946

PXD042946 is an original dataset announced via ProteomeXchange.

Title	Metaproteomic assessment of gut microbial and host functional perturbations in Helicobacter pylori-infected patients subjected to an antimicrobial protocol
Description	The impact of therapeutic interventions on the human gut microbiota (GM) is a clinical issue of paramount interest given the strong interconnection between microbial dynamics and human health. Orally administered antibiotics are known to reduce GM biomass and modify GM taxonomic profile. However, the impact of antimicrobial therapies on GM functions and biochemical pathways has scarcely been studied. Here, we characterized the fecal metaproteome of 10 Helicobacter pylori-infected patients before (T0) and after 10 days (T1) of a successful quadruple therapy (bismuth, tetracycline, metronidazole, and rabeprazole) and 30 days after therapy cessation (T2), to investigate how GM and host functions change during the eradication and healing processes. At T1, the abundance ratio between microbial and host proteins was reversed compared with that at T0 and T2. Several pathobionts (including Klebsiella, Proteus, Enterococcus, Muribaculum, and Enterocloster) were increased at T1. Therapy reshaped the relative contributions of the functions required to produce acetate, propionate, and butyrate. Proteins related to the uptake and processing of complex glycans were increased. Microbial cross-feeding with sialic acid, fucose, and rhamnose was enhanced, whereas hydrogen sulfide production was reduced. Finally, microbial proteins involved in antibiotic resistance and inflammation were more abundant after therapy. Moreover, a reduction in host proteins with known roles in inflammation and H. pylori-mediated carcinogenesis was observed. In conclusion, our results support the use of metaproteomics to monitor drug-induced remodeling of GM and host functions, opening the way for investigating new antimicrobial therapies aimed at preserving gut environmental homeostasis.
HostingRepository	PRIDE
AnnounceDate	2023-12-14
AnnouncementXML	Submission_2023-12-14_01:23:04.223.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alessandro Tanca
SpeciesList	scientific name: human gut metagenome; NCBI TaxID: 408170; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-13 09:16:53	ID requested
⏵ 1	2023-12-14 01:23:07	announced

10.1080/19490976.2023.2291170;

Abbondio M, Tanca A, De Diego L, Sau R, Bibb, ò S, Pes GM, Dore MP, Uzzau S, -infected patients subjected to an antimicrobial protocol. Gut Microbes, 15(2):2291170(2023) [pubmed]

submitter keyword: antibiotics,metaproteomics, Helicobacter, mass spectrometry, gut microbiota, human microbiome

Sergio Uzzau
contact affiliation	Department of Biomedical Sciences, University of Sassari, Italy
contact email	uzzau@uniss.it
lab head
Alessandro Tanca
contact affiliation	Department of Biomedical Sciences, University of Sassari, Sassari, Sardinia
contact email	aletanca@uniss.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042946
     1. Label: PRIDE project
     2. Name: Metaproteomic assessment of gut microbial and host functional perturbations in Helicobacter pylori-infected patients subjected to an antimicrobial protocol