PXD042940

PXD042940 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of extracellular vesicle released by MCF7 cells in the absence of the tetraspanins CD9, CD81 or CD63
Description	The tetraspanins CD9, CD81, and CD63 are among the most widely used markers of Extracellular Vesicles (EVs) and are also used to isolate the vesicles they decorate. If their function in EVs is largely unknown, they have been suggested to play a role in the sorting of protein cargos into EVs or to regulate the uptake of EVs by acceptor cells. Using different tetraspanin-deficient MCF7 breast cancer cell lines, we have shown that the absence of any of these 3 tetraspanins had a minimal impact on their protein composition, as analyzed by label-free quantitative mass spectrometry. However, the EVs released by cells deficient in both CD9 and CD81 showed a decrease level of CD9P-1/EWI-F and EWI-2 (encoded by the genes PTGFRN and IGSF8 respectively), which are two well-characterized CD9 and CD81 molecular partners.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:59:40.257.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD042940
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Cédric Pionneau
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-13 03:47:16	ID requested
1	2023-08-03 02:09:53	announced
2	2023-11-14 06:58:28	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:59:40	announced	2024-10-22: Updated project metadata.

10.6019/PXD042940;

10.1002/JEV2.12352;

submitter keyword: tetraspanins, exosomes, MCF7, PTGFRN, mass spectrometry, CD9,Extracellular vesicles, CD81, IgSF8, CD63, proteome, timsTOF Pro

Eric RUBINSTEIN
contact affiliation	Sorbonne Université, Inserm, CNRS, Centre d'Immunologie et des Maladies Infectieuses, CIMI-Paris, F-75013 Paris, France
contact email	eric.rubinstein@inserm.fr
lab head
Cédric Pionneau
contact affiliation	Plateforme P3S UMS-37 PASS – Sorbonne Universite
contact email	cedric.pionneau@sorbonne-universite.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD042940

PRIDE project URI

• PRIDE
  1. PXD042940
     1. Label: PRIDE project
     2. Name: Proteomic analysis of extracellular vesicle released by MCF7 cells in the absence of the tetraspanins CD9, CD81 or CD63