PXD042918

PXD042918 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Quantitative proteomic analysis of MCM3 in ThinPrep samples of patients with cervical preinvasive cancer
Description	Triage methods for cervical cancer detection show moderate accuracy and present considerable false-negative and false-positive result rates. A complementary diagnostic parameter could help improve the accuracy of identifying patients who need treatment. A pilot study was performed using a targeted proteomics approach with opportunistic ThinPrep samples obtained from women collected at the hospital’s outpatient clinic to determine the concentration levels of minichromosome maintenance-3 (MCM3) and envoplakin (EVPL) proteins. Forty samples with ’negative for intraepithelial lesion or malignancy’ (NILM), 21 samples with ’atypical squamous cells of undetermined significance’ (ASC-US), and 33 samples with ’low-grade squamous intraepithelial lesion and worse’ (≥LSIL) were analyzed, using cytology and the patients’ histology reports. Highly accurate concordance was obtained for gold standard-confirmed samples, demonstrating that the MCM3/EVPL ratio can discriminate between non-dysplastic and dysplastic samples. On that account, we propose that MCM3 and EVPL are promising candidate protein biomarkers for population-based cervical cancer screening.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:11:39.665.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD042918
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Coskun Guzel
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Orbitrap Exploris 120

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-12 14:14:38	ID requested
1	2023-10-24 13:23:14	announced
⏵ 2	2023-11-14 09:11:45	announced	2023-11-14: Updated project metadata.

Publication List

K, ö, se B, Laar RV, Beekhuizen HV, Kemenade FV, Baykal AT, Luider T, G, ü, zel C, Quantitative Proteomic Analysis of MCM3 in ThinPrep Samples of Patients with Cervical Preinvasive Cancer. Int J Mol Sci, 24(13):(2023) [pubmed]
10.6019/PXD042918;

K, ö, se B, Laar RV, Beekhuizen HV, Kemenade FV, Baykal AT, Luider T, G, ü, zel C, Quantitative Proteomic Analysis of MCM3 in ThinPrep Samples of Patients with Cervical Preinvasive Cancer. Int J Mol Sci, 24(13):(2023) [pubmed]

10.6019/PXD042918;

Keyword List

submitter keyword: Human, PRM, MCM3, Cervical Cancer

submitter keyword: Human, PRM, MCM3, Cervical Cancer

Contact List

Theo Luider
contact affiliation	Head of Laboratory Neuro-Oncology, Clinical and Cancer Proteomics, Neurology
contact email	t.luider@erasmusmc.nl
lab head
Coskun Guzel
contact affiliation	Erasmus MC
contact email	c.guzel@erasmusmc.nl
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD042918
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD042918

PRIDE project URI

Repository Record List

[ + ]