PXD042900

PXD042900 is an original dataset announced via ProteomeXchange.

Title	AP-1B regulates interaction between intraepithelial lymphocyte-epithelial cell in the intestine
Description	Intraepithelial lymphocytes (IELs) reside in the epithelial layer to protect against foreign pathogens and maintain epithelial barrier functions in the intestine. The interaction between IELs and epithelial cells is required for their functions; however, the underlying molecular machinery still remains to be elucidated. Here, we found that intestinal epithelium-specific deficiency in a clathrin adaptor protein (AP)-1B, which regulates the basolateral protein sorting, led to the massive reduction of IELs. Quantitative proteomics demonstrated that dozens of proteins, including known IEL-interacting proteins—E-cadherin, Btnl2, and Plexin B2—decreased in the basolateral membrane of AP-1B-deficient epithelial cells. Among the downregulated proteins, CD166 is included. CD166 interacted with CD6 on the surface of induced IELs. CD166 knockdown using shRNA in intestinal organoid culture significantly inhibited the recruitment of IELs to an epithelial layer. These data suggest that basolateral sorting by AP-1B is indispensable for maintaining IELs in the epithelial layer and survival of IELs.
HostingRepository	jPOST
AnnounceDate	2024-09-26
AnnouncementXML	Submission_2024-09-26_05:13:28.489.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kosuke Ogata
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; TMT6plex reporter+balance reagent N-acylated residue; TMT6plex reporter+balance reagent acylated N-terminal; Acetyl
Instrument	instrument

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-12 03:29:35	ID requested
⏵ 1	2024-09-26 05:13:28	announced

Dataset with its publication pending

submitter keyword: intraepithelial lymphocyte-epithelial cell, AP-1B

Koji Hase
lab head
Kosuke Ogata
contact affiliation	Kyoto university
dataset submitter

jPOST dataset URI

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