PXD042891

PXD042891 is an original dataset announced via ProteomeXchange.

Title	SWATH-MS analysis of blood plasma and circulating small extracellular vesicles enables detection of putative protein biomarkers of fertility in Young and Aged dairy cows.
Description	The development of biomarkers of fertility could provide benefits for the genetic improvement of dairy cows. Circulating small extracellular vesicles (sEVs) show promise as diagnostic or prognostic markers since their cargo reflects the metabolic state of the cell of origin; thus, they mirror the physiological status of the host. Here, we employed data-independent acquisition mass spectrometry to survey the plasma and plasma sEV proteomes of two different cohorts of Young (Peripubertal; n = 30) and Aged (Primiparous; n = 20) dairy cows (Bos taurus) of high- and low-genetic merit of fertility and known pregnancy outcomes (ProteomeXchange dataset identifier PXD042891). We established predictive models of fertility status, with an area under the curve of 0.97 (sEV; p value = 3.302e-07) and 0.95 (plasma; p value = 6.405e-08). Biomarker candidates unique to high-fertility Young cattle had a sensitivity of 0.77 and specificity of 0.67 (*p = 0.0287). Low-fertility biomarker candidates uniquely identified in sEVs from Young and Aged cattle had a sensitivity and specificity of 0.69 and 1.0, respectively (***p = 0.0005). Our bioinformatics pipeline enabled quantification of plasma and circulating sEV proteins associated with fertility phenotype. Further investigations are warranted to validate this research in a larger population, which may lead to improved classification of fertility status in cattle.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:28:32.797.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Natalie Turner
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-11 21:35:49	ID requested
1	2023-10-14 14:32:11	announced
⏵ 2	2023-11-14 08:28:33	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: fertility, extracellular vesicle, exosome, Bos taurus, cattle, dairy cow, bioinformatics,SWATH, data-independent acquisition, biomarker, reproduction

Murray D.
contact affiliation	Faculty of Health School of Biomedical Sciences Queensland University of Technology
contact email	murray.mitchell@qut.edu.au
lab head
Natalie Turner
contact affiliation	Queensland University of Technology
contact email	natalie.turner@hdr.qut.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042891
     1. Label: PRIDE project
     2. Name: SWATH-MS analysis of blood plasma and circulating small extracellular vesicles enables detection of putative protein biomarkers of fertility in Young and Aged dairy cows.