PXD042829 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Assembly of cellular proteins with the HIV-1 genome into viral particles regulate virus production and infectivity |
| Description | The initial steps of the human immunodeficiency virus 1 (HIV-1) lifecycle are regulated by cellular RNA-binding proteins (RBPs). However, the scope of these early host-virus interactions remain largely uncharecterised. To fill this gap, we developed in virion RNA interactome capture (ivRIC) to profile comprehensively and systematically the proteins that interact with the HIV-1 genomic (g)RNA inside viral particles. ivRIC identified 105 cellular RBPs within the encapsidated HIV-1 ribonucleoprotein, many of which are surprisingly nuclear. Analysis of the interactome of HIV-1 RBP Rev in infected cells revealed that most in virion incorporated (iv)RBPs likely associate with HIV-1 gRNA during its nuclear life. Functional assays showed that ivRBPs are important for HIV-1. For example, we found that PURA and PURB control viral gene expression and infectivity through interaction with critical sequences in the gRNA. In summary, the compositional analysis of the encapsidated ribonucleoprotein of HIV-1 uncovers host-virus interactions controlling virus infection. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-05-25 |
| AnnouncementXML | Submission_2026-05-24_17:01:53.031.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Honglin Chen |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: HIV-1 vector pNL4-3; NCBI TaxID: NEWT:151458; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2023-06-08 17:45:58 | ID requested | |
| ⏵ 1 | 2026-05-24 17:01:53 | announced | |
Publication List
| 10.1016/j.celrep.2026.117090; |
| Garcia-Moreno M, Embarc-Buh A, Truman R, Noerenberg M, Iselin L, Chen H, Lenz CE, Lee JY, Dicker K, Shetty SD, Palmalux N, Gu Q, Sohier TJM, J, รค, rvelin AI, Kamel W, Ruscica V, Ricci EP, Davis I, Mohammed S, Castello A, Incorporation of genome-bound cellular proteins into HIV-1 particles regulates viral infection. Cell Rep, 45(4):117090(2026) [pubmed] |
Keyword List
| submitter keyword: HIV |
| RNA |
| RNA-binding protein |
| virus infection |
| virion |
| viral particle |
| Rev |
| RNA-interactome capture |
| PURA |
| PURB |
Contact List
| Alfredo Castello |
|---|
| contact affiliation | Centre for Virus Research, University of Glasgow |
| contact email | Alfredo.Castello@glasgow.ac.uk |
| lab head | |
| Honglin Chen |
|---|
| contact affiliation | Centre for Virus Research, University of Glasgow |
| contact email | 2722555c@student.gla.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD042829 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD042829
- Label: PRIDE project
- Name: Assembly of cellular proteins with the HIV-1 genome into viral particles regulate virus production and infectivity
