PXD042825

PXD042825 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Native Nanoproteomics Captures Structure and Dynamics of Endogenous Protein Complexes in Human Heart Tissue
Description	To obtain a molecular-level understanding of biological processes, it is critical to perform structural characterization of protein complexes and their subunits directly from human tissues. Native top-down mass spectrometry (MS) has emerged as a complementary technique to traditional structural biology methods. Nonetheless, native top-down MS has primarily characterized recombinant or high-abundance protein complexes, as strategies to enrich and preserve low-abundance protein complexes from human tissues is lacking. Here we have developed a native nanoproteomics platform integrating peptide-functionalized nanoparticles to characterize low-abundance protein complexes with high-resolution native top-down MS. We then apply our native nanoproteomics strategy to enrich and structurally elucidate endogenous troponin (cTn) complexes directly from human heart tissue. Our results not only reveal the native complex assembly, post-translational modification landscape, and Ca2+ binding dynamics of the cTn complex but also enable us to propose a paradigm for structural and dynamical characterization of endogenous native protein complexes from human tissues.
HostingRepository	MassIVE
AnnounceDate	2023-11-06
AnnouncementXML	Submission_2023-11-06_06:43:02.384.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Emily Chapman
SpeciesList	scientific name: Human;
ModificationList	Phospho
Instrument	timsTOF Pro; solariX; impact; maXis

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-08 09:53:45	ID requested
⏵ 1	2023-11-06 06:43:02	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Native top-down, Mass spectrometry, Endogenous protein complex, cardiac troponin, nanoproteomics

submitter keyword: Native top-down, Mass spectrometry, Endogenous protein complex, cardiac troponin, nanoproteomics

Contact List

Ying Ge
contact affiliation	University of Wisconsin - Madison
contact email	ying.ge@wisc.edu
lab head
Emily Chapman
contact affiliation	University of Wisconsin - Madison
contact email	eachapman2@wisc.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000092130/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000092130/