PXD042800

PXD042800 is an original dataset announced via ProteomeXchange.

Title	The let-7 family of miRNA mediates paternal intergenerational inheritance of metabolic disorders via post-transcriptional regulation of mitochondrial dynamics.
Description	This study aims to investigate the molecular mechanisms and nature of impaired soma-germline crosstalk in IEI by focusing on the miRNA regulations in soma and sperm of obese male mice. We found that F0 HFD deteriorated glucose metabolism in F0 and F1, whereas F0 weight loss largely corrected glucose metabolism in both generations. F0 and F1 mice from HFD and REV group exhibited eWAT hypertrophy, and increased Leptin and insulin levels. Transcriptomics and proteomics revealed metabolic dysfunction in F0 and F1 eWAT which correlated with dysregulation of mitochondrial genes. Notably, miRNA Let-7 was obesity-induced in eWAT and sperm, and mice sired from Let-7d/e-microinjected embryos showed impaired glucose metabolism, suggesting Let-7 participated in IEI of metabolic disorders. Our data demonstrated that paternal obesity-induced pre-diabetes and mitochondrial dysfunction in eWAT correlated with similar observations in offspring and the phenomena could be partially precluded by paternal weight-loss before conception.
HostingRepository	PRIDE
AnnounceDate	2026-01-06
AnnouncementXML	Submission_2026-01-06_07:25:40.862.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Proteomics Facility
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	monohydroxylated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-07 09:55:22	ID requested
⏵ 1	2026-01-06 07:25:41	announced

Dataset with its publication pending

submitter keyword: metabolic diseases, mitochondrial dysfunction,microRNA, intergenerational epigenetic inheritance, Let-7

Aleksandra Trifunovic
contact affiliation	Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD) and Institute for Mitochondrial Diseases and Aging, Medical Faculty, University of Cologne, Cologne, Germany.
contact email	aleksandra.trifunovic@uk-koeln.de
lab head
Proteomics Facility
contact affiliation	CECAD Research Center
contact email	proteomics-facility@uni-koeln.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD042800

PRIDE project URI

• PRIDE
  1. PXD042800
     1. Label: PRIDE project
     2. Name: The let-7 family of miRNA mediates paternal intergenerational inheritance of metabolic disorders via post-transcriptional regulation of mitochondrial dynamics.