PXD042798
PXD042798 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Wolbachia strains differ in arbovirus inhibition mechanisms in Aedes mosquitoes |
| Description | Some strains of the inherited bacterium Wolbachia have been shown to be effective at reducing the transmission of dengue and other positive-sense RNA viruses by Aedes aegypti in both laboratory and field settings and are being deployed for dengue control. The degree of virus inhibition varies between Wolbachia strains; density and tissue tropism can contribute to these differences but there are also indications that this is not the only factor involved: for example, strains wAu and wAlbA are maintained at similar densities but only wAu produces strong dengue inhibition. We previously reported perturbations in lipid transport dynamics, including sequestration of cholesterol in lipid droplets, with strains wMel / wMelPop in Ae. aegypti. Here we show that strain wAu does not produce the same cholesterol sequestration phenotype despite displaying strong virus inhibition and moreover, in contrast to wMel, wAu antiviral activity was not rescued by cyclodextrin treatment. To further investigate the cellular basis underlying these differences, proteomic analysis of midguts was carried out on Ae. aegypti lines and revealed that wAu-carrying midguts showed a distinct proteome when compared to Wolbachia-free, wMel- or wAlbA-carrying midguts, in particular with respect to lipid transport and metabolism. The data suggest a possible role for perturbed RNA processing pathways in wAu virus inhibition. Together these results indicate that wAu shows unique features in its inhibition of arboviruses compared to previously characterized Wolbachia strains |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-02-15 |
| AnnouncementXML | Submission_2024-02-15_15:19:39.326.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Adam Dowle |
| SpeciesList | scientific name: Wolbachia; NCBI TaxID: 953; scientific name: Aedes aegypti; common name: yellow fever mosquito; NCBI TaxID: 7159; |
| ModificationList | TMT6plex; Oxidation; Carbamidomethyl |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-06-07 09:11:04 | ID requested | |
| ⏵ 1 | 2024-02-15 15:19:40 | announced |
Publication List
| Rainey SM, Geoghegan V, Lefteri DA, Ant TH, Martinez J, McNamara CJ, Kamel W, de Laurent ZR, Castello A, Sinkins SP, Differences in proteome perturbations caused by the Wolbachia strain wAu suggest multiple mechanisms of Wolbachia-mediated antiviral activity. Sci Rep, 13(1):11737(2023) [pubmed] |
Keyword List
| submitter keyword: Wolbachia, wAu, wAlbA, wMel, Aedes aegypti, mosquito, arbovirus, DENV, ZIKV, midgut, cyclodextrin |
Contact List
| Professor Steven Sinkins | |
|---|---|
| contact affiliation | University of Glasgow |
| contact email | Steven.Sinkins@glasgow.ac.uk |
| lab head | |
| Adam Dowle | |
| contact affiliation | University of York |
| contact email | adam.dowle@york.ac.uk |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v05/MSV000092124/ |
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