PXD042773

PXD042773 is an original dataset announced via ProteomeXchange.

Title	Mechanism of orphan subunit recognition during assembly quality control_BioMass@LMB
Description	Cells contain numerous abundant molecular machines assembled from multiple subunits. Imbalances in subunit production and failed assembly generate orphan subunits that are eliminated by poorly defined pathways. Here, we determined how orphan subunits of the cytosolic chaperonin CCT are recognized. Several unassembled CCT subunits recruited the E3 ubiquitin ligase HERC2 using ZNRD2 as an adaptor. Both factors were necessary for orphan CCT subunit degradation in cells, sufficient for CCT subunit ubiquitination with purified factors, and necessary for optimal cell fitness. Domain mapping and structure prediction defined the molecular features of a minimal HERC2-ZNRD2-CCT module. The structural model, whose key elements were validated in cells using point mutants, shows why ZNRD2 selectively recognizes multiple orphaned CCT subunits without engaging assembled CCT. Our findings reveal how failures during CCT assembly are monitored and provide a paradigm for the molecular recognition of orphan subunits, the largest source of quality control substrates in cells.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_06:44:25.703.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Catarina Franco
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-06 12:40:48	ID requested
1	2023-07-26 07:33:24	announced
⏵ 2	2023-11-14 06:44:26	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: chaperonin CCT, subunit composition

Ramanujan S. Hegde
contact affiliation	MRC Laboratory of Molecular Biology, Cambridge, United Kingdom
contact email	rhegde@mrc-lmb.cam.ac.uk
lab head
Catarina Franco
contact affiliation	MRC Laboratory of Molecular Biology
contact email	cffranco@mrc-lmb.cam.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042773
     1. Label: PRIDE project
     2. Name: Mechanism of orphan subunit recognition during assembly quality control_BioMass@LMB