PXD042769

PXD042769 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	LC-MS of control vs BafA1-treated mouse oocytes during maturation
Description	We discovered that mouse oocytes contain novel non-membrane-bound organelles, which we named EndoLysosomal Vesicular Assemblies (ELVAs). ELVAs contain endolysosomal and autophagy vesicles, as well as proteasomes. In immature oocytes, ELVAs store ubiquitinated proteins and show little degradative activity. During oocyte maturation, ELVAs increase protein degradation, thereby degrading the stored proteins. To understand which proteins are being degraded inside ELVAs during oocyte maturation we treated mouse oocytes with or without the lysosomal inhibitor Bafilomycin A1 (BafA1) during maturation and subjected equal number of in vitro matured oocytes to mass spec, looking for enriched proteins in the BafA1-treated sample.
HostingRepository	PRIDE
AnnounceDate	2024-02-20
AnnouncementXML	Submission_2024-02-20_03:15:22.532.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gabriele Zaffagnini
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-06 10:56:06	ID requested
⏵ 1	2024-02-20 03:15:22	announced

Publication List

10.1016/J.CELL.2024.01.031;

10.1016/J.CELL.2024.01.031;

Keyword List

submitter keyword: oocyte, lysosome, maturation, mouse, protein degradation

submitter keyword: oocyte, lysosome, maturation, mouse, protein degradation

Contact List

Elvan Böke
contact affiliation	Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Barcelona, Spain.
contact email	elvan.boke@crg.eu
lab head
Gabriele Zaffagnini
contact affiliation	Centre for Genomic Regulation
contact email	gabriele.zaffagnini@crg.eu
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD042769
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD042769

PRIDE project URI

Repository Record List

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