⮝ Full datasets listing

PXD042765

PXD042765 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome and phospholipidome interrelationship of synovial fluid-derived extracellular vesicles in Equine osteoarthritis: An exploratory ‘multi-omic’ study
DescriptionOsteoarthritis (OA) causes persistent joint deterioration, severe morbidity, and reduced mobility in both humans and horses. Extracellular vesicles (EVs) are key players in cell-to-cell communication and are thus ideal for biomarker discovery. Here, we used a unique multi-omics strategy to find possible EV biomarkers from the synovial fluid of healthy horses compared to naturally occurring OA and advanced OA. We discovered that SF-EVs’ proteome and phospholipidome are correlated. Furthermore, we found that the quantity of EVs present in SF was unaffected by OA. We discovered crucial signalling pathways involving integrins (p=2.72x10-24), the actin cytoskeleton (p=3.88x10-32), Rho family GTPases (p=1.24x10-19), and the clathrin-mediated endocytosis pathway (p=1.30x10-24). Specifically, the advanced OA group’s immunological response (macrophage binding and interaction) was upregulated. These findings are suggestive that the membrane and protein cargo of SF-EVs are altered in response to OA pathology and may be used as biomarkers of disease. Consequently, SF-EVs could have the potential to be employed in the future to help with OA stratification and prognosis. In addition, this study provides a framework for future research using a larger cohort.
HostingRepositoryPRIDE
AnnounceDate2024-05-24
AnnouncementXMLSubmission_2024-05-24_03:11:19.631.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRosalind Jenkins
SpeciesList scientific name: Equus caballus (Horse); NCBI TaxID: 9796;
ModificationListiodoacetamide derivatized residue
InstrumentTripleTOF 6600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-06-06 09:05:35ID requested
12024-05-24 03:11:20announced
Publication List
10.1016/j.bbrep.2023.101635;
Clarke E, Varela L, Jenkins RE, Lozano-Andr, é, s E, Cywi, ń, ska A, Przewozny M, van Weeren PR, van de Lest CHA, Peffers M, Wauben MHM, Proteome and phospholipidome interrelationship of synovial fluid-derived extracellular vesicles in equine osteoarthritis: An exploratory 'multi-omics' study to identify composite biomarkers. Biochem Biophys Rep, 37():101635(2024) [pubmed]
Keyword List
submitter keyword: proteomics, lipidomics, extracellular vesicles, equine, synovial fluid,Osteoarthritis
Contact List
Rosalind Elspeth Jenkins
contact affiliationCDSS Bioanalytical Facility, Pharmacology & Therapeutics, LIV-SRF, University of Liverpool, UK
contact emailR.Jenkins@liv.ac.uk
lab head
Rosalind Jenkins
contact affiliationUniversity of Liverpool
contact emailR.Jenkins@liv.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD042765
PRIDE project URI
Repository Record List
[ + ]