PXD042765 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteome and phospholipidome interrelationship of synovial fluid-derived extracellular vesicles in Equine osteoarthritis: An exploratory ‘multi-omic’ study |
Description | Osteoarthritis (OA) causes persistent joint deterioration, severe morbidity, and reduced mobility in both humans and horses. Extracellular vesicles (EVs) are key players in cell-to-cell communication and are thus ideal for biomarker discovery. Here, we used a unique multi-omics strategy to find possible EV biomarkers from the synovial fluid of healthy horses compared to naturally occurring OA and advanced OA. We discovered that SF-EVs’ proteome and phospholipidome are correlated. Furthermore, we found that the quantity of EVs present in SF was unaffected by OA. We discovered crucial signalling pathways involving integrins (p=2.72x10-24), the actin cytoskeleton (p=3.88x10-32), Rho family GTPases (p=1.24x10-19), and the clathrin-mediated endocytosis pathway (p=1.30x10-24). Specifically, the advanced OA group’s immunological response (macrophage binding and interaction) was upregulated. These findings are suggestive that the membrane and protein cargo of SF-EVs are altered in response to OA pathology and may be used as biomarkers of disease. Consequently, SF-EVs could have the potential to be employed in the future to help with OA stratification and prognosis. In addition, this study provides a framework for future research using a larger cohort. |
HostingRepository | PRIDE |
AnnounceDate | 2024-05-24 |
AnnouncementXML | Submission_2024-05-24_03:11:19.631.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Rosalind Jenkins |
SpeciesList | scientific name: Equus caballus (Horse); NCBI TaxID: 9796; |
ModificationList | iodoacetamide derivatized residue |
Instrument | TripleTOF 6600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-06-06 09:05:35 | ID requested | |
⏵ 1 | 2024-05-24 03:11:20 | announced | |
Publication List
10.1016/j.bbrep.2023.101635; |
Clarke E, Varela L, Jenkins RE, Lozano-Andr, é, s E, Cywi, ń, ska A, Przewozny M, van Weeren PR, van de Lest CHA, Peffers M, Wauben MHM, Proteome and phospholipidome interrelationship of synovial fluid-derived extracellular vesicles in equine osteoarthritis: An exploratory 'multi-omics' study to identify composite biomarkers. Biochem Biophys Rep, 37():101635(2024) [pubmed] |
Keyword List
submitter keyword: proteomics, lipidomics, extracellular vesicles, equine, synovial fluid,Osteoarthritis |
Contact List
Rosalind Elspeth Jenkins |
contact affiliation | CDSS Bioanalytical Facility, Pharmacology & Therapeutics, LIV-SRF, University of Liverpool, UK |
contact email | R.Jenkins@liv.ac.uk |
lab head | |
Rosalind Jenkins |
contact affiliation | University of Liverpool |
contact email | R.Jenkins@liv.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD042765
- Label: PRIDE project
- Name: Proteome and phospholipidome interrelationship of synovial fluid-derived extracellular vesicles in Equine osteoarthritis: An exploratory ‘multi-omic’ study