PXD042765

PXD042765 is an original dataset announced via ProteomeXchange.

Title	Proteome and phospholipidome interrelationship of synovial fluid-derived extracellular vesicles in Equine osteoarthritis: An exploratory ‘multi-omic’ study
Description	Osteoarthritis (OA) causes persistent joint deterioration, severe morbidity, and reduced mobility in both humans and horses. Extracellular vesicles (EVs) are key players in cell-to-cell communication and are thus ideal for biomarker discovery. Here, we used a unique multi-omics strategy to find possible EV biomarkers from the synovial fluid of healthy horses compared to naturally occurring OA and advanced OA. We discovered that SF-EVs’ proteome and phospholipidome are correlated. Furthermore, we found that the quantity of EVs present in SF was unaffected by OA. We discovered crucial signalling pathways involving integrins (p=2.72x10-24), the actin cytoskeleton (p=3.88x10-32), Rho family GTPases (p=1.24x10-19), and the clathrin-mediated endocytosis pathway (p=1.30x10-24). Specifically, the advanced OA group’s immunological response (macrophage binding and interaction) was upregulated. These findings are suggestive that the membrane and protein cargo of SF-EVs are altered in response to OA pathology and may be used as biomarkers of disease. Consequently, SF-EVs could have the potential to be employed in the future to help with OA stratification and prognosis. In addition, this study provides a framework for future research using a larger cohort.
HostingRepository	PRIDE
AnnounceDate	2024-05-24
AnnouncementXML	Submission_2024-05-24_03:11:19.631.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rosalind Jenkins
SpeciesList	scientific name: Equus caballus (Horse); NCBI TaxID: 9796;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-06 09:05:35	ID requested
⏵ 1	2024-05-24 03:11:20	announced

10.1016/j.bbrep.2023.101635;

Clarke E, Varela L, Jenkins RE, Lozano-Andr, é, s E, Cywi, ń, ska A, Przewozny M, van Weeren PR, van de Lest CHA, Peffers M, Wauben MHM, Proteome and phospholipidome interrelationship of synovial fluid-derived extracellular vesicles in equine osteoarthritis: An exploratory 'multi-omics' study to identify composite biomarkers. Biochem Biophys Rep, 37():101635(2024) [pubmed]

submitter keyword: proteomics, lipidomics, extracellular vesicles, equine, synovial fluid,Osteoarthritis

Rosalind Elspeth Jenkins
contact affiliation	CDSS Bioanalytical Facility, Pharmacology & Therapeutics, LIV-SRF, University of Liverpool, UK
contact email	R.Jenkins@liv.ac.uk
lab head
Rosalind Jenkins
contact affiliation	University of Liverpool
contact email	R.Jenkins@liv.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042765
     1. Label: PRIDE project
     2. Name: Proteome and phospholipidome interrelationship of synovial fluid-derived extracellular vesicles in Equine osteoarthritis: An exploratory ‘multi-omic’ study