Updated project metadata. A novel MNT-1 cell line deficient in NPC1 protein was generated using CRISPR/CAS9 knock-out method. In the absence of the NPC1 protein the pigmentation status of the cells was changed and alterations in the melanogenesis process were observed. In order to characterize the role of NPC1 protein in the pigmentation process we compared the proteome of the MNT-1 WT cell line with the NPC1-KO cell line.