PXD042747

PXD042747 is an original dataset announced via ProteomeXchange.

Title	S-acylproteomic analysis of HeLa cells by nGF-SPE
Description	S-acylation is an essential lipidation form in eukaryotes, in which various fatty acids can be covalently attached to specific cysteine residues. Here, we developed a pretreatment method for direct separation and global analysis of endogenously intact S-acylated peptides by nanographite fluoride-based solid-phase extraction (nGF-SPE), together with the investigation and optimization of enrichment procedure as well as LC-MS/MS analysis process. Consequently, we performed the first global profiling of endogenously intact S-acylated peptides, with 701 S-palmitoylated peptides from HeLa cell lysates in restricted search. Furthermore, coupling the nGF-SPE method with open search mode, altogether 1119 intact S-acylated peptides were identified with the attached palmitate, palmitoleate, myristate and octanoic chain respectively, providing a global insight to the endogenously heterogeneous modification state. S-acylproteome were separated at flow rate of 300 nL/min on a nanoElute chromatography system (Bruker Daltonics) with the IonOptick column (AURORA Series column, 75 µm i.d. × 25 cm, C18, 1.6 μm) and then analyzed by a hybrid trapped ion mobility spectrometry quadrupole time of-flight mass spectrometer (timsTOF Pro, Bruker Daltonics) via data-dependent acquisition (DDA) mode. Obtained raw data was analyzed with PEAKS Online.
HostingRepository	iProX
AnnounceDate	2023-06-05
AnnouncementXML	Submission_2023-06-05_20:35:28.362.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guanghui Ji
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	S-palmitoyl-L-cysteine; S-acylated residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-05 20:34:59	ID requested
⏵ 1	2023-06-05 20:35:28	announced

Dataset with its publication pending

submitter keyword: LC-MS/MS, Intact S-acylated peptides, S-acylproteomic analysis

Haojie Lu
contact affiliation	Department of Chemistry, Fudan University
contact email	luhaojie@fudan.edu.cn
lab head
Guanghui Ji
contact affiliation	Department of Chemistry, Fudan University
contact email	17110220008@fudan.edu.cn
dataset submitter

iProX dataset URI