PXD042733

PXD042733 is an original dataset announced via ProteomeXchange.

Title	Identification of cellular protein interacting with HBV genome by protein mass spectrometry
Description	: Hepatitis B virus (HBV) remains a major public health threat with more than 296 million people chronically infected worldwide at high risk to develop hepatocellular carcinoma. Current therapies are effective in suppressing HBV replication but rarely lead to cure. The fundamental limitation rests on the fact that current therapies do not affect the HBV covalently closed circular DNA (cccDNA), which serves as the template for viral transcription and replication and is highly stable in infected cells to ensure viral persistence. In this study, we aim to identify and elucidate the functional role of cccDNA-associated host factors using affinity purification and protein mass spectrometry in HBV-infected cells. HBV core protein (HBc) is associated with cccDNA. We used an anti-HBc antibody to pull down HBcAg-associated cccDNA complex in nuclear extract of HBV-infected HepG2-NTCP cells. By protein mass spectrometry, we identified many host proteins, including previously known proteins, that are associated with HBV cccDNA. Some of these proteins were functionally validated to play a role in HBV cccDNA activities.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:45:35.235.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD042733
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	David Anderson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-05 10:50:05	ID requested
1	2024-06-16 01:10:14	announced
⏵ 2	2024-10-22 06:45:36	announced	2024-10-22: Updated project metadata.

10.6019/PXD042733;

Xia Y, Cheng X, Nilsson T, Zhang M, Zhao G, Inuzuka T, Teng Y, Li Y, Anderson DE, Holdorf M, Liang TJ, Nucleolin binds to and regulates transcription of hepatitis B virus covalently closed circular DNA minichromosome. Proc Natl Acad Sci U S A, 120(49):e2306390120(2023) [pubmed]

10.1073/pnas.2306390120;

submitter keyword: HBV cccDNA, mini-chromosome, transcription, therapy

T. Jake Liang
contact affiliation	Chief, Liver Diseases Branch, NIDDK, NIH, DHHS, U.S. Government
contact email	jakel@bdg10.niddk.nih.gov
lab head
David Anderson
contact affiliation	NIH/NIDDK
contact email	davida@mail.nih.gov
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD042733

PRIDE project URI

• PRIDE
  1. PXD042733
     1. Label: PRIDE project
     2. Name: Identification of cellular protein interacting with HBV genome by protein mass spectrometry