PXD042731

PXD042731 is an original dataset announced via ProteomeXchange.

Title	Characterization of clumpy adhesion of Escherichia coli to human cells and associated factors influencing antibiotic sensitivity
Description	Escherichia coli intestinal infection pathotypes are characterized by distinct adhesion patterns including the recently described clumpy adhesion phenotype. Here we identify and characterize genetic factors contributing to clumpy adhesion of E. coli strain 4972. In this strain, the transcriptome and proteome of adhered bacteria were found distinct from planktonic bacteria in supernatant. A total of 622 genes in the transcriptome were differentially expressed in bacteria present in clumps relative to the planktonic bacteria. Seven genes targeted for disruption had variable distribution in different pathotypes and nonpathogenic E. coli with pilV and spnT genes being the least frequent or absent from most groups. Deletion (Δ) of five differentially expressed genes, flgH, ffp, pilV, spnT and yggT affected motility, adhesion or antibiotic stress. ΔflgH exhibited 80 % decrease and ΔyggT depicted 145.5 % increase in adhesion, and upon complementation, adhesion significantly reduced to 13 %. ΔflgH lost motility and was regenerated when complemented whereas Δffp had significantly increased motility and reintroduction of the same gene reduced it to the wild-type level. The clumps produced by of Δffp and ΔspnT were more resistant and protected the bacteria with ΔspnT showing the best clump formation in terms of ampicillin stress protection. ΔyggT had the lowest tolerance to gentamicin where the antibiotic stress completely eliminated the bacteria. Overall, we were able to investigate the influence of clump formation on the cell surface adhesion and antimicrobial tolerance with the contribution of several factors crucial to clump formation on susceptibility to the selected antibiotics.
HostingRepository	PRIDE
AnnounceDate	2024-04-09
AnnouncementXML	Submission_2024-04-09_06:10:49.906.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christina Ludwig
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-05 09:22:00	ID requested
⏵ 1	2024-04-09 06:10:50	announced

10.1128/spectrum.02606-23;

Khan MM, Sidorczuk K, Becker J, Aleksandrowicz A, Baraniewicz K, Ludwig C, Ali A, Kingsley RA, Schierack P, Kolenda R, to human cells and associated factors influencing antibiotic sensitivity. Microbiol Spectr, 12(5):e0260623(2024) [pubmed]

ProteomeXchange project tag: EPIC-XS

submitter keyword: Escherichia coli, clumpy adhesion, motility, stress, antibiotic tolerance

Christina Ludwig
contact affiliation	Bavarian Center for Biomolecular Mass Spectrometry (BayBioMS) School of Life Sciences Technical University of Munich (TUM) Gregor-Mendel-Straße 4 85354 Freising GERMANY
contact email	tina.ludwig@tum.de
lab head
Christina Ludwig
contact affiliation	TU Munich
contact email	tina.ludwig@tum.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042731
     1. Label: PRIDE project
     2. Name: Characterization of clumpy adhesion of Escherichia coli to human cells and associated factors influencing antibiotic sensitivity