PXD042724

PXD042724 is an original dataset announced via ProteomeXchange.

Title	Molecular basis for the enzymatic inactivity of class III glutaredoxins on standard glutathionylated substrates
Description	Glutaredoxins (GRXs) are small proteins that interact with the atypical tripeptide glutathione (GSH), a redox active metabolite that forms a disulfide (GSSG) upon oxidation. GRXs encoding variants of a CPYC motif at a conserved active site act as GSH-dependent thiol-disulfide oxidoreductases (class I). GRXs with a CGFS site (class II) bind GSH in a way that is non-permissive for thiol-disulfide oxidoreductase reactions and favours transient iron-sulfur cluster binding. The biochemical functions of CCxC/S-type (class III) GRXs, which are found only in land plants, have remained largely unexplored. In this study, we characterized the in vitro properties of one of the Arabidopsis thaliana class III GRXs, namely ROXY9.
HostingRepository	PRIDE
AnnounceDate	2025-01-14
AnnouncementXML	Submission_2025-01-14_03:55:38.774.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gereon Poschmann
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	half cystine; N-ethylmaleimide derivatized cysteine; L-cysteine sulfinic acid; acetylated residue; monohydroxylated residue; L-cysteic acid (L-cysteine sulfonic acid); L-cysteine glutathione disulfide
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-05 01:54:20	ID requested
⏵ 1	2025-01-14 03:55:39	announced

Mrozek P, Grunewald S, Treffon K, Poschmann G, Rabe von Pappenheim F, Tittmann K, Gatz C, Molecular basis for the enzymatic inactivity of class III glutaredoxin ROXY9 on standard glutathionylated substrates. Nat Commun, 16(1):589(2025) [pubmed]

10.1038/s41467-024-55532-z;

submitter keyword: Arabidopsis thaliana, glutathione, redox potential, thiol-disulfide, CC-type glutaredoxins, oxidoreductase

Gereon Poschmann
contact affiliation	Proteome Research Institute of Molecular Medicine Heinrich Heine University Düsseldorf 40225 Düsseldorf Germany
contact email	gereon.poschmann@hhu.de
lab head
Gereon Poschmann
contact affiliation	Molecular Proteomics Laboratory
contact email	gereon.poschmann@hhu.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042724
     1. Label: PRIDE project
     2. Name: Molecular basis for the enzymatic inactivity of class III glutaredoxins on standard glutathionylated substrates