PXD042723

PXD042723 is an original dataset announced via ProteomeXchange.

Title	Bacterial symbionts use a type VI secretion system for fitness increase against pathobionts
Description	The Type VI Secretion System (T6SS) in bacteria is a versatile mechanism that facilitates protein transport into neighboring cells and can act as an antibacterial weapon by eliminating competing organisms in the vicinity. The objective of this study was to characterize the T6SS in Aggregatibacter aphrophilus and assess its antimicrobial capabilities through competition with Aggregatibacter actinomycetemcomitans in a multispecies biofilm. The proteomic analysis consisted of two parts, referred to as monospecies biofilm and multispecies biofilms, respectively. Initially, we examined the protein profiles of monospecies biofilms formed by two strains of Aggregatibacter aphrophilus, namely HK83 and CCUG 11575, along with their Hcp mutant derivatives (Hcp being a core protein for T6SS). Each strain was analyzed with six replicates (n=4 for HK83, HK83 hcp, CCUG 11575, and CCUG 11575 hcp). Subsequently, the HK83 and CCUG 11575 strains, as well as their Hcp mutant derivatives, were individually introduced into a multispecies biofilm. This multispecies biofilm consisted of seven species, namely A. actinomycetemcomitans JP2 strain (OMZ 295), Actinomyces oris (OMZ 745), Candida albicans (OMZ 110), Fusobacterium nucleatum subsp. nucleatum KP-F2 (OMZ 598), Streptococcus oralis SK248 (OMZ 607), Streptococcus mutans UA159 (OMZ 918), and Veillonella dispar ATCC 17748T (OMZ 493). These species were selected to mimic the natural co-habitat of A. aphrophilus and A. actinomycetemcomitans. Furthermore, control 7-species biofilms with A. aphrophilus strains HK83, HK83 hcp, CCUG 11575, and CCUG 11575 hcp (n=4 each) underwent proteomic analysis to gain insights into the protein expression and potential interactions within the biofilm community.
HostingRepository	PRIDE
AnnounceDate	2024-05-24
AnnouncementXML	Submission_2024-05-24_03:10:02.530.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kai Bao
SpeciesList	scientific name: Actinomyces oris; NCBI TaxID: 544580; scientific name: Candida albicans (Yeast); NCBI TaxID: 5476; scientific name: Aggregatibacter aphrophilus; NCBI TaxID: 732; scientific name: Streptococcus oralis; NCBI TaxID: 1303; scientific name: Veillonella dispar; NCBI TaxID: 39778; scientific name: Aggregatibacter actinomycetemcomitans (Actinobacillus actinomycetemcomitans) (Haemophilus actinomycetemcomitans); NCBI TaxID: 714; scientific name: Streptococcus mutans; NCBI TaxID: 1309; scientific name: Fusobacterium nucleatum subsp. nucleatum; NCBI TaxID: 76856;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetic acid derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-05 01:26:01	ID requested
⏵ 1	2024-05-24 03:10:03	announced

10.1016/j.isci.2024.109650;

submitter keyword: Aggregatibacter actinomycetemcomitans,T6SS, Aggregatibacter aphrophilus

Nagihan Bostanci
contact affiliation	Department of Dental Medicine (DENTMED), OF, Karolinska Institutet
contact email	nagihan.bostanci@ki.se
lab head
Kai Bao
contact affiliation	postdoctoral researcher
contact email	kai.bao@ki.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042723
     1. Label: PRIDE project
     2. Name: Bacterial symbionts use a type VI secretion system for fitness increase against pathobionts