PXD042723 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Bacterial symbionts use a type VI secretion system for fitness increase against pathobionts |
Description | The Type VI Secretion System (T6SS) in bacteria is a versatile mechanism that facilitates protein transport into neighboring cells and can act as an antibacterial weapon by eliminating competing organisms in the vicinity. The objective of this study was to characterize the T6SS in Aggregatibacter aphrophilus and assess its antimicrobial capabilities through competition with Aggregatibacter actinomycetemcomitans in a multispecies biofilm. The proteomic analysis consisted of two parts, referred to as monospecies biofilm and multispecies biofilms, respectively. Initially, we examined the protein profiles of monospecies biofilms formed by two strains of Aggregatibacter aphrophilus, namely HK83 and CCUG 11575, along with their Hcp mutant derivatives (Hcp being a core protein for T6SS). Each strain was analyzed with six replicates (n=4 for HK83, HK83 hcp, CCUG 11575, and CCUG 11575 hcp). Subsequently, the HK83 and CCUG 11575 strains, as well as their Hcp mutant derivatives, were individually introduced into a multispecies biofilm. This multispecies biofilm consisted of seven species, namely A. actinomycetemcomitans JP2 strain (OMZ 295), Actinomyces oris (OMZ 745), Candida albicans (OMZ 110), Fusobacterium nucleatum subsp. nucleatum KP-F2 (OMZ 598), Streptococcus oralis SK248 (OMZ 607), Streptococcus mutans UA159 (OMZ 918), and Veillonella dispar ATCC 17748T (OMZ 493). These species were selected to mimic the natural co-habitat of A. aphrophilus and A. actinomycetemcomitans. Furthermore, control 7-species biofilms with A. aphrophilus strains HK83, HK83 hcp, CCUG 11575, and CCUG 11575 hcp (n=4 each) underwent proteomic analysis to gain insights into the protein expression and potential interactions within the biofilm community. |
HostingRepository | PRIDE |
AnnounceDate | 2024-05-24 |
AnnouncementXML | Submission_2024-05-24_03:10:02.530.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Kai Bao |
SpeciesList | scientific name: Actinomyces oris; NCBI TaxID: 544580; scientific name: Candida albicans (Yeast); NCBI TaxID: 5476; scientific name: Aggregatibacter aphrophilus; NCBI TaxID: 732; scientific name: Streptococcus oralis; NCBI TaxID: 1303; scientific name: Veillonella dispar; NCBI TaxID: 39778; scientific name: Aggregatibacter actinomycetemcomitans (Actinobacillus actinomycetemcomitans) (Haemophilus actinomycetemcomitans); NCBI TaxID: 714; scientific name: Streptococcus mutans; NCBI TaxID: 1309; scientific name: Fusobacterium nucleatum subsp. nucleatum; NCBI TaxID: 76856; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetic acid derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-06-05 01:26:01 | ID requested | |
⏵ 1 | 2024-05-24 03:10:03 | announced | |
Publication List
Oscarsson J, Bao K, Shiratsuchi A, Grossmann J, Wolski W, Aung KM, Lindholm M, Johansson A, Mowsumi FR, Wai SN, Belibasakis GN, Bostanci N, Bacterial symbionts in oral niche use type VI secretion nanomachinery for fitness increase against pathobionts. iScience, 27(5):109650(2024) [pubmed] |
10.1016/j.isci.2024.109650; |
Keyword List
submitter keyword: Aggregatibacter actinomycetemcomitans,T6SS, Aggregatibacter aphrophilus |
Contact List
Nagihan Bostanci |
contact affiliation | Department of Dental Medicine (DENTMED), OF, Karolinska Institutet |
contact email | nagihan.bostanci@ki.se |
lab head | |
Kai Bao |
contact affiliation | postdoctoral researcher |
contact email | kai.bao@ki.se |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD042723
- Label: PRIDE project
- Name: Bacterial symbionts use a type VI secretion system for fitness increase against pathobionts