PXD042711

PXD042711 is an original dataset announced via ProteomeXchange.

Title	Immunopeptidome of a human beta cell line infected by Coxsackievirus B strains
Description	Coxsackievirus B (CVB) infection of pancreatic β cells is associated with β-cell autoimmunity. We investigated how CVB impacts human β cells and anti-CVB T-cell responses. β cells were efficiently infected by CVB in vitro, downregulated HLA Class I and presented few, selected HLA-bound viral peptides. Circulating CD8+ T cells from CVB-seropositive individuals recognized only a fraction of these peptides, and only another sub-fraction was targeted by effector/memory T cells that expressed the exhaustion marker PD-1. T cells recognizing a CVB epitope cross-reacted with the GAD β-cell antigen. Infected β cells, which formed filopodia to propagate infection, were more efficiently killed by CVB than by anti-CVB T cells. Thus, our in-vitro and ex-vivo data suggest limited T-cell responses to CVB, supporting the rationale for CVB vaccination trials for type 1 diabetes prevention. CVB-reactive CD8+ T cells provide biomarkers to follow response to infection and vaccination.
HostingRepository	PRIDE
AnnounceDate	2023-08-19
AnnouncementXML	Submission_2023-08-18_16:57:01.016.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	RobertoMallone
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-04 09:13:34	ID requested
⏵ 1	2023-08-18 16:57:01	announced

Dataset with its publication pending

submitter keyword: Coxsackievirus, beta cells,immunopeptidome

RobertoMallone
contact affiliation	Université Paris Cité, Institut Cochin, CNRS, INSERM, Paris, France
contact email	roberto.mallone@inserm.fr
lab head
RobertoMallone
contact affiliation	Université Paris Cité, Institut Cochin, CNRS, INSERM, Paris
contact email	roberto.mallone@inserm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD042711

PRIDE project URI

• PRIDE
  1. PXD042711
     1. Label: PRIDE project
     2. Name: Immunopeptidome of a human beta cell line infected by Coxsackievirus B strains