PXD042682

PXD042682 is an original dataset announced via ProteomeXchange.

Title	Proteomics and bioinformatics investigations to improve serological diagnosis of canine brucellosis
Description	Brucella canis is pathogenic for dogs and humans. Serological diagnosis is a cost-effective approach for disease surveillance, but a major drawback of current serological tests is the cross-reactivity with other bacteria that results in false positive reactions, and development of indirect tests with improved sensitivity and specificity remain a priority. A western blotting assay was developed to define the serum antibody patterns associated to infection using a panel of positive and negative dog sera. B. canis positive sera recognized immunogenic bands ranging from 7 to 30 kDa that were then submitted to ESI–LC-MS/MS and analyzed by bioinformatics tools. A total of 398 B. canis proteins were identified. Bioinformatics tools identified 16 non cytoplasmic immunogenic proteins predicted as non-homologous with the most important Brucella cross-reactive bacteria and 9 B. canis proteins non-homologous to B. ovis; among the latter, one resulted non-homologous to B. melitensis. The western blotting test developed was able to distinguish between infected and non-infected animals and may serve as confirmatory test for the serological diagnosis of B. canis. The mass spectrometry and in silico results lead to the identification of specific candidate antigens that pave the way for the development of more accurate indirect diagnostic tests
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:10:40.401.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD042682
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ivanka Krasteva
SpeciesList	scientific name: Brucella canis ATCC 23365; NCBI TaxID: 483179;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-02 09:18:55	ID requested
1	2023-10-24 13:01:23	announced
⏵ 2	2023-11-14 09:10:41	announced	2023-11-14: Updated project metadata.

Luciani M, Krasteva I, Di Febo T, Perletta F, D'Onofrio F, De Massis F, D'Alterio N, Sacchini F, Tittarelli M, Proteomics and bioinformatics investigations to improve serological diagnosis of canine brucellosis. Proteomics Clin Appl, 17(6):e2200116(2023) [pubmed]

10.6019/PXD042682;

submitter keyword: Brucella canis

Western blotting

Mass spectrometry

Protein identification,Bioinformatics

Mirella Luciani
contact affiliation	Istituto zooprofilattico dell'abruzzo e del molise
contact email	m.luciani@izs.it
lab head
Ivanka Krasteva
contact affiliation	Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "G. Caporale"
contact email	i.krasteva@izs.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042682
     1. Label: PRIDE project
     2. Name: Proteomics and bioinformatics investigations to improve serological diagnosis of canine brucellosis