PXD042681

PXD042681 is an original dataset announced via ProteomeXchange.

Title	Sequence-specific binding between HOTAIR and PURB reveals a new mode for LncRNA-mediated regulation of p53
Description	Here we identify PURB, a dual DNA/RNA-binding protein, as a key mediator for LncRNAs to interact with p53. PURB is overexpressed in human cancers and loss of PURB expression in human cancer cells induces tumor growth suppression by activating p53. Interestingly, PURB knockdown activates only a subset of p53 target genes without affecting p53 protein levels and PURB is specifically recruited by p53 to the target genes in a promoter-specific manner. Moreover, a unique cis-regulatory element is identified at the target promoters recognized by PURB; loss of this element does not affect p53-mediated transactivation but abrogates the ability of p53 to recruit PURB to the promoters for repression. Notably, the ability of PURB in transcriptional repression requires its sequence-specific binding with HOTAIR, one of LncRNAs tightly associated with PURB. HOTAIR interacts directly with SUV39H1 and, bridging by the PURB/HOTAIR complex, p53 is able to recruit SUV39H1 histone methyltransferase to the target promoters for transcriptional repression. These data establish a new mode of LncRNA-mediated regulation of p53 transcription in a sequence-specific manner and also reveal, a previously unanticipated mechanism for promoter-specific regulation through a unique cis-regulatory element recognized by the LncRNA-protein complex.
HostingRepository	PRIDE
AnnounceDate	2025-04-24
AnnouncementXML	Submission_2025-04-23_18:30:41.270.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD042681
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Zhangchuan Xia
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-02 09:05:31	ID requested
⏵ 1	2025-04-23 18:30:41	announced

10.6019/PXD042681;

submitter keyword: long non-coding RNA, cis-regulatory element, epigenetics, promoter-specific regulation, HOTAIR, SUV39H1, tumor suppression, PURB,p53

Dr. Wei Gu
contact affiliation	Department of Pathology and Cell Biology and Institute for Cancer Genetics Herbert Irving Comprehensive Cancer Vagelos College of Physicians & Surgeons Columbia University (lab head)
contact email	wg8@cumc.columbia.edu
lab head
Zhangchuan Xia
contact affiliation	Institute for Cancer Genetics Herbert Irving Comprehensive Cancer Center Vagelos College of Physicians & Surgeons Columbia University
contact email	zx2264@cumc.columbia.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042681
     1. Label: PRIDE project
     2. Name: Sequence-specific binding between HOTAIR and PURB reveals a new mode for LncRNA-mediated regulation of p53