PXD042668

PXD042668 is an original dataset announced via ProteomeXchange.

Title	Protein Extraction from Ancient Teeth and Dental Calculus Compatible with Subsequent DNA Extraction
Description	Dental calculus is becoming a crucial material in the study of past populations, with increasing interest in its proteomic and genomic content. Here we suggest further development of protocol for analysis of ancient proteins and a combined approach for subsequent ancient DNA extraction. We tested the protocol on recent teeth. We then applied the optimised protocol to ancient teeth to limit the destruction of calculus, as it is a precious and irreplaceable source of dietary, microbiological, and ecological information in the archaeological context. Finally, the applicability of the protocol was proven on samples of ancient calculus.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:28:29.401.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pavel Roudnický
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro; Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-02 07:34:04	ID requested
1	2023-09-11 00:24:12	announced
⏵ 2	2023-11-14 08:28:29	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: protein extraction, palaeoproteomics, ancient DNA, ancient proteins, dental calculus,bioarchaeology

Zbyněk Zdráhal
contact affiliation	Proteomics Core Facility and Research Group Proteomics, CEITEC-MU, Masaryk University, Brno, Czech Republic
contact email	zdrahal@sci.muni.cz
lab head
Pavel Roudnický
contact affiliation	Masaryk University
contact email	p.roudnicky@mail.muni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD042668

PRIDE project URI

• PRIDE
  1. PXD042668
     1. Label: PRIDE project
     2. Name: Protein Extraction from Ancient Teeth and Dental Calculus Compatible with Subsequent DNA Extraction