PXD042662 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Pseudouridine guides germline small RNA transport and epigenetic inheritance |
| Description | Epigenetic modifications that arise during plant and animal development, such as DNA and histone modification, are mostly reset during gamete formation, but some are inherited from the germline including those marking imprinted genes. Small RNAs guide these epigenetic modifications, and some are also inherited by the next generation. In C. elegans, inherited small RNA precursors have poly (UG) tails, but how inherited small RNAs are distinguished in other animals and plants is unknown. Pseudouridine (Ψ) is the most abundant RNA modification but has not been explored in small RNAs. Here, we develop novel assays to detect Ψ in short RNA sequences, demonstrating its presence in mouse and Arabidopsis microRNAs and their precursors. We also detect substantial enrichment in germline small RNAs, namely epigenetically activated siRNAs (easiRNAs) in Arabidopsis pollen, and piwi-interacting piRNAs in mouse testis. In pollen, pseudouridylated easiRNAs are localized to sperm cells, and we found that PAUSED/HEN5 (PSD), the plant homolog of Exportin-t, interacts genetically with Ψ and is required for transport of easiRNAs into sperm cells from the vegetative nucleus. We further show that Exportin-t is required for the triploid block: chromosome dosage-dependent seed lethality that is epigenetically inherited from pollen. Thus, Ψ has a conserved role in marking inherited small RNAs in the germline. |
| HostingRepository | PRIDE |
| AnnounceDate | 2024-04-29 |
| AnnouncementXML | Submission_2024-04-29_07:10:40.085.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD042662 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Agata Malinowska |
| SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
| ModificationList | methylthiolated residue; phosphorylated residue; monohydroxylated residue |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2023-06-02 07:26:24 | ID requested | |
| ⏵ 1 | 2024-04-29 07:10:40 | announced | |
Publication List
Keyword List
| submitter keyword: pseudouridine,small RNA, epigenetic inheritance |
Contact List
| Mateusz Michal Bajczyk |
|---|
| contact affiliation | Department of Gene Expression, Faculty of Biology, Adam Mickiewicz University in Poznan, Poznan, Poland |
| contact email | mateusz.bajczyk@amu.edu.pl |
| lab head | |
| Agata Malinowska |
|---|
| contact affiliation | IBB PAS |
| contact email | esme@ibb.waw.pl |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD042662
- Label: PRIDE project
- Name: Pseudouridine guides germline small RNA transport and epigenetic inheritance
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