PXD042660

PXD042660 is an original dataset announced via ProteomeXchange.

Title	Exploring extracellular vesicles of probiotic yeast Saccharomyces boulardii CNCM I-745 as carriers of biologically active molecules transferred to human intestinal cells
Description	Extracellular vesicles (EVs) are nanoparticles containing various bioactive cargos, e.g., proteins, RNAs, and lipids, that are released into the environment by all cell types. They are involved in, amongst others, intercellular communication. This article presents studies on EVs produced by the probiotic yeast Saccharomyces boulardii CNCM I-745. The size distribution and concentration of EVs in the liquid culture of yeast were estimated. Moreover, the vesicles of S. boulardii were tested for their cytotoxicity against three model human intestinal cell lines. This study did not show any significant negative effect of yeast EVs on these cells under tested conditions. In addition, EVs of S. boulardii were verified for their ability to internalize in vitro with human cells and transfer their cargo. The yeast vesicles were loaded with doxorubicin, an anticancer agent, and added to the cellular cultures. Subsequently, microscopic observations revealed that these EVs transferred the chemotherapeutic to human intestinal cell lines. A cytotoxicity test confirmed the activity of the transferred doxorubicin. Detailed information about the proteins present in EVs might be important in terms of exploring yeast EVs as carriers of active molecules. Thus, proteomic analysis of the EV content was also conducted within the present study, and it allowed to identify 541 proteins after matching to the Saccharomyces Genome Database (SGD). Altogether, this study provides strong evidence that the EVs of the probiotic CNCM I-745 strain could be considered a drug delivery system.
HostingRepository	PRIDE
AnnounceDate	2023-06-29
AnnouncementXML	Submission_2023-06-29_13:16:41.573.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD042660
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	EwaSitkiewicz
SpeciesList	scientific name: Saccharomyces boulardii (nom. inval.); NCBI TaxID: 252598;
ModificationList	methylthiolated residue; monohydroxylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-02 07:25:20	ID requested
⏵ 1	2023-06-29 13:16:41	announced

10.6019/PXD042660;

submitter keyword: doxorubicin,extracellular vesicles, Saccharomyces boulardii, bioactive molecules, EVs, probiotic yeasts, drug carriers

JolantaMierzejewska
contact affiliation	Chair of Drug and Cosmetics Biotechnology, Faculty of Chemistry Warsaw University of Technology, Poland
contact email	jolanta.mierzejewska@pw.edu.pl
lab head
EwaSitkiewicz
contact affiliation	Mass Spectrometry Laboratory Institute of Biochemistry and Biophysics Polish Academy of Sciences
contact email	ewa@ibb.waw.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042660
     1. Label: PRIDE project
     2. Name: Exploring extracellular vesicles of probiotic yeast Saccharomyces boulardii CNCM I-745 as carriers of biologically active molecules transferred to human intestinal cells