PXD042648

PXD042648 is an original dataset announced via ProteomeXchange.

Title	Deciphering the proteome of naturally released extracellular vesicles from Leptospira interrogans serovar Pomona in response to temperature and osmotic stresses
Description	The biogenesis and functions of Leptospira's extracellular vesicles (EVs) remain unclear. The study aimed to characterize EVs that were naturally released from intact leptospires during in vitro culture, as well as under conditions mimicking the host environment during infection, including temperature or osmolarity shift. The EVs were found to contain a group of proteins from the outer membrane and various cytoplasmic proteins, suggesting that they originated from the outer membrane and cytoplasm. These proteins were primarily involved in Leptospira's growth, survival, adaptation, and pathogenicity. Interestingly, the study revealed that Leptospira secreted multifunctional proteins through EVs but might maintain several virulence and virulence-associated proteins in their cells in response to temperature and osmotic stresses. Thus, native leptospiral EVs might act as decoys for the host immune system, while the virulence factors crucial for direct interaction with host components might be preserved in whole cells. This knowledge may contribute to understanding the role of EVs in the pathogenicity of Leptospira and the development of vaccines against leptospirosis in the future.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:27:37.282.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jiradej Makjaroen
SpeciesList	scientific name: Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130; NCBI TaxID: 267671;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-02 03:15:47	ID requested
1	2024-01-26 10:41:43	announced
⏵ 2	2024-10-22 06:27:38	announced	2024-10-22: Updated project metadata.

10.1038/s41598-023-45863-0;

Techawiwattanaboon T, Phanchamnan E, Iadsee N, Makjaroen J, Pisitkun T, Patarakul K, Proteomic profile of naturally released extracellular vesicles secreted from Leptospira interrogans serovar Pomona in response to temperature and osmotic stresses. Sci Rep, 13(1):18601(2023) [pubmed]

submitter keyword: Quantitative proteomics, Extracellular vesicle, Leptospirosis, Osmotic stress,Leptospira

Kanitha Patarakul
contact affiliation	Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Pathumwan, Bangkok, Thailand
contact email	Kanitha.Pa@chula.ac.th
lab head
Jiradej Makjaroen
contact affiliation	Center of Excellence in Systems Biology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330
contact email	jiradejmak@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD042648

PRIDE project URI

• PRIDE
  1. PXD042648
     1. Label: PRIDE project
     2. Name: Deciphering the proteome of naturally released extracellular vesicles from Leptospira interrogans serovar Pomona in response to temperature and osmotic stresses