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PXD042648

PXD042648 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDeciphering the proteome of naturally released extracellular vesicles from Leptospira interrogans serovar Pomona in response to temperature and osmotic stresses
DescriptionThe biogenesis and functions of Leptospira's extracellular vesicles (EVs) remain unclear. The study aimed to characterize EVs that were naturally released from intact leptospires during in vitro culture, as well as under conditions mimicking the host environment during infection, including temperature or osmolarity shift. The EVs were found to contain a group of proteins from the outer membrane and various cytoplasmic proteins, suggesting that they originated from the outer membrane and cytoplasm. These proteins were primarily involved in Leptospira's growth, survival, adaptation, and pathogenicity. Interestingly, the study revealed that Leptospira secreted multifunctional proteins through EVs but might maintain several virulence and virulence-associated proteins in their cells in response to temperature and osmotic stresses. Thus, native leptospiral EVs might act as decoys for the host immune system, while the virulence factors crucial for direct interaction with host components might be preserved in whole cells. This knowledge may contribute to understanding the role of EVs in the pathogenicity of Leptospira and the development of vaccines against leptospirosis in the future.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:27:37.282.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJiradej Makjaroen
SpeciesList scientific name: Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130; NCBI TaxID: 267671;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-06-02 03:15:47ID requested
12024-01-26 10:41:43announced
22024-10-22 06:27:38announced2024-10-22: Updated project metadata.
Publication List
10.1038/s41598-023-45863-0;
Techawiwattanaboon T, Phanchamnan E, Iadsee N, Makjaroen J, Pisitkun T, Patarakul K, Proteomic profile of naturally released extracellular vesicles secreted from Leptospira interrogans serovar Pomona in response to temperature and osmotic stresses. Sci Rep, 13(1):18601(2023) [pubmed]
Keyword List
submitter keyword: Quantitative proteomics, Extracellular vesicle, Leptospirosis, Osmotic stress,Leptospira
Contact List
Kanitha Patarakul
contact affiliationDepartment of Microbiology, Faculty of Medicine, Chulalongkorn University, Pathumwan, Bangkok, Thailand
contact emailKanitha.Pa@chula.ac.th
lab head
Jiradej Makjaroen
contact affiliationCenter of Excellence in Systems Biology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330
contact emailjiradejmak@gmail.com
dataset submitter
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Dataset FTP location
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