PXD042648 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Deciphering the proteome of naturally released extracellular vesicles from Leptospira interrogans serovar Pomona in response to temperature and osmotic stresses |
Description | The biogenesis and functions of Leptospira's extracellular vesicles (EVs) remain unclear. The study aimed to characterize EVs that were naturally released from intact leptospires during in vitro culture, as well as under conditions mimicking the host environment during infection, including temperature or osmolarity shift. The EVs were found to contain a group of proteins from the outer membrane and various cytoplasmic proteins, suggesting that they originated from the outer membrane and cytoplasm. These proteins were primarily involved in Leptospira's growth, survival, adaptation, and pathogenicity. Interestingly, the study revealed that Leptospira secreted multifunctional proteins through EVs but might maintain several virulence and virulence-associated proteins in their cells in response to temperature and osmotic stresses. Thus, native leptospiral EVs might act as decoys for the host immune system, while the virulence factors crucial for direct interaction with host components might be preserved in whole cells. This knowledge may contribute to understanding the role of EVs in the pathogenicity of Leptospira and the development of vaccines against leptospirosis in the future. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:27:37.282.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jiradej Makjaroen |
SpeciesList | scientific name: Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130; NCBI TaxID: 267671; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-06-02 03:15:47 | ID requested | |
1 | 2024-01-26 10:41:43 | announced | |
⏵ 2 | 2024-10-22 06:27:38 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1038/s41598-023-45863-0; |
Techawiwattanaboon T, Phanchamnan E, Iadsee N, Makjaroen J, Pisitkun T, Patarakul K, Proteomic profile of naturally released extracellular vesicles secreted from Leptospira interrogans serovar Pomona in response to temperature and osmotic stresses. Sci Rep, 13(1):18601(2023) [pubmed] |
Keyword List
submitter keyword: Quantitative proteomics, Extracellular vesicle, Leptospirosis, Osmotic stress,Leptospira |
Contact List
Kanitha Patarakul |
contact affiliation | Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Pathumwan, Bangkok, Thailand |
contact email | Kanitha.Pa@chula.ac.th |
lab head | |
Jiradej Makjaroen |
contact affiliation | Center of Excellence in Systems Biology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330 |
contact email | jiradejmak@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD042648
- Label: PRIDE project
- Name: Deciphering the proteome of naturally released extracellular vesicles from Leptospira interrogans serovar Pomona in response to temperature and osmotic stresses