FFPE tissue specimens from 54 patients who underwent surgery at the Affiliated Hospital of Jiangnan University were selected for the study.we employed FFPE slides from CRC, CRLM, and HCC patients to undertake rapid proteomics analysis. Subsequently, we conducted differential protein intensity and functional analyses based on the proteomics results. We constructed a machine learning model to simplify the diagnostic model and employed immunohistochemistry to validate the CRLM and HCC diagnosis detection markers.