PXD042634

PXD042634 is an original dataset announced via ProteomeXchange.

Title	Fasciola gigantica excretory and secretory products co-immunoprecipitated using secondary infected buffalo sera shows different component to that of primary infection
Description	Introduction: The secondary infection of F. gigantica 4 weeks after primary infection could not induce a robust adaptive immune response in buffalo. As excretory and secretory products (ESP) show intense immune regulation and plays critical role in primary infection process, while research on ESP in secondary infection is lacking. Does the components of ESP in secondary infection was different from that of primary infection? Does fluke secrete and excrete new component to immunoregulation during secondary infection? Here, the component of F. gigantica ESP in buffalo primary and secondary infections was identified and compared, which will drive the exploration in repeat infection in basis matter, and also conducive to drug targets and vaccine candidate screening. Methods: Buffaloes were assigned to the group A (n = 3, non-infection), group B (n = 3, primary infection) and group C (n = 3, secondary infection). The primary infection group was orally administrated 250 metacercariae at 4 week post-infection (wpi), while the secondary infection group was orally administrated 250 metacercariae at 0 and 4 wpi. Then Sera were collected at 0, 10, and 16 wpi for group A, 0 (pre-infection), 1, 3, 6, 8, 10, 13, and 16 wpi for group B, and 0, 1, 3, 4, 5, 7, 10, 12, 14, 17 and 20 wpi for group C. FgESP components interacting with sera from three groups were pulled down by co-immunoprecipitated (co-IP) and identified using LC–MS/MS. Interacting FgESP components in group C were subjected to Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathway and gene ontology (GO) functional annotation to infer their potential functions, and also been compared with that of group B. Results and discussion: In the group C, 327 proteins were identified, of which 74 were consistently identified at 7 time points (5, 7, 10, 12, 14, 17 and 20 wpi). GO analysis shows these 74 proteins mainly functioned in biological processes, while KEGG analysis showed they mainly functioned in metabolism and cellular processing. The numbers of specific interactors identified were 2 (5 wpi), 10 (7 wpi), 0 (10 wpi), 8 (12 wpi), 1 (14 wpi), 4 (17 wpi), and 4 (20 wpi). Here, the antigenic targets in FgESP in the secondary infection process were screened over a long and intensive period of time, and these components needs further exploration. The present study will deepen the understanding of molecular F. gigantica-buffalo interactions and helpful in new potential vaccine and drug target candidates identification
HostingRepository	iProX
AnnounceDate	2023-06-01
AnnouncementXML	Submission_2023-08-31_19:19:19.209.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xinping Kong
SpeciesList	scientific name: Bubalus bubalis; NCBI TaxID: 89462; scientific name: Fasciola gigantica; NCBI TaxID: 46835;
ModificationList	S-carboxamidomethyl-L-cysteine
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-01 18:53:52	ID requested
⏵ 1	2023-08-31 19:19:19	announced

Dataset with its publication pending

submitter keyword: Fasciola gigantica, LC–MS/MS, co-immunoprecipitation, excretory and secretory products, interaction, screening

Wenda Di
contact affiliation	Guangxi University
contact email	diwenda@gxu.edu.cn
lab head
Xinping Kong
contact affiliation	Gungxi University
contact email	kxp3802@163.com
dataset submitter

iProX dataset URI