PXD042630 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Control pull-downs testing the effect of histone native chemical ligation on protein binding to di-nucleosomes |
Description | Here we examined whether histones H3 and H4 produced using native chemical ligation reaction affect protein binding to di-nucleosomes. To test this we performed a set of affinity purification pull-downs using di-nucleosomes containing the following histones H3 and H4: 1. ligated unmodified histone H3 and recombinant wild type histone H4 (unmod. H3), 2. recombinant wild type histone H3 and ligated unmodified histone H4 (unmod. H4) 3. ligated unmodified histones H3 and H4 (unmod. H3&H4). 4. recombinant wild type histones H3 and H4 (WT) Each pull-down was performed in three replicates. Co-purified proteins were quantified using label-free MS. Note that ligated histones H3 and H4 contain T32C or I29C single amino acid substitutions, respectively, that are introduced by the native chemical ligation procedure. In addition, ligated histone H4 is N-terminally acetylated to mimic its naturally blocked N-terminus. |
HostingRepository | PRIDE |
AnnounceDate | 2023-12-10 |
AnnouncementXML | Submission_2023-12-10_12:27:53.659.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Andrey Tvardovskiy |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-06-01 15:12:18 | ID requested | |
⏵ 1 | 2023-12-10 12:27:54 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: nucleosome pull-down,native chemical ligation |
Contact List
Till Bartke |
contact affiliation | Institute of Functional Epigenetics, Helmholtz Zentrum München, 85764 Neuherberg, Germany. |
contact email | till.bartke@helmholtz-muenchen.de |
lab head | |
Andrey Tvardovskiy |
contact affiliation | Institute of Functional Epigenetics, Helmholtz Zentrum München, 85764 Neuherberg, Germany |
contact email | tvardovsky.a@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD042630
- Label: PRIDE project
- Name: Control pull-downs testing the effect of histone native chemical ligation on protein binding to di-nucleosomes