PXD042607

PXD042607 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomic dataset comparing wild type, bob1-1 and bob1-1dbf4∆ cells in M-phase arrested S.cerevisiae.
Description	DNA double-strand breaks (DSBs) can be repaired by several pathways. In eukaryotes, repair pathway choice – the cellular decision making underlying DSB repair – occurs at the level of DSB resection and is controlled by the cell cycle. Upon cell cycle-dependent activation, cyclin-dependent kinases (CDKs) phosphorylate resection proteins and thereby stimulate DSB resection and repair by homologous recombination (HR). We uncovered a novel role for the Dbf4-dependent kinase Cdc7 (DDK) in regulating HR and DNA end resection. We therefore analyzed phosphorylation substrates of DDK by phosphoproteomics, where we compared wild type, bob1-1 and bob1-1dbf4∆ cells in M-phase arrested S.cerevisiae.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:30:07.522.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Barbara Steigenberger
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-01 03:25:53	ID requested
1	2024-02-15 11:18:09	announced
⏵ 2	2024-10-22 06:30:07	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: DDK, Dbf4-Cdc7, cell-cycle,Saccharomyces cerevisiae

Prof. Dr. Boris Pfander
contact affiliation	Institute of Aerospace Medicine German Aerospace Center (DLR) Linder Höhe 51147 Cologne, Germany Phone: +49-2203-6011-277 Email: boris.pfander@dlr.de & Institute for Genome Stability in Ageing and Disease University of Cologne CECAD Research Center Joseph-Stelzmann-Str. 26 50931 Cologne, Germany Email: bpfander@uni-koeln.de & Max-Planck Institute of Biochemistry, Am Klopferspitz 18 82152 Martinsried, Germany Phone:+49-89-8578-3050 Fax: +49-89-8578-2454, Email: bpfander@biochem.mpg.de
contact email	bpfander@biochem.mpg.de
lab head
Barbara Steigenberger
contact affiliation	MPI of Biochemistry
contact email	steigenberger@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD042607

PRIDE project URI

• PRIDE
  1. PXD042607
     1. Label: PRIDE project
     2. Name: Phosphoproteomic dataset comparing wild type, bob1-1 and bob1-1dbf4∆ cells in M-phase arrested S.cerevisiae.