PXD042605

PXD042605 is an original dataset announced via ProteomeXchange.

Title	The fungal pathogen Ustilago maydis targets the maize corepressor TPL2 to modulate host transcription for tumorigenisis
Description	Ustilago maydis is a biotrophic fungus that causes tumor formation on all aerial parts of maize. U. maydis secretes effector proteins during penetration and colonization to successfully overcome the plant immune response and reprogram host physiology to promote infection. In this study, we functionally characterized the U. maydis effector protein Topless (TPL) interacting protein 6 (Tip6). We found that Tip6 interacts with the N-terminus of ZmTPL2 through its two EAR (Ethylene-responsive element binding factor-associated amphiphilic repression) motifs. We show that the EAR motifs are essential for the virulence function of Tip6 and critical for altering the nuclear distribution pattern of ZmTPL2. We propose that Tip6 mimics the recruitment of ZmTPL2 by plant repressor proteins, thus disrupting host transcriptional regulation. We show that a large group of AP2/ERF B1 subfamily transcription factors are misregulated in the presence of Tip6. Our study suggests a regulatory mechanism where the U. maydis effector Tip6 utilizes repressive domains to recruit the corepressor ZmTPL2 to disrupt the transcriptional networks of the host plant.
HostingRepository	PRIDE
AnnounceDate	2023-11-09
AnnouncementXML	Submission_2023-11-09_05:57:13.497.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sara ChristinaStolze
SpeciesList	scientific name: Nicotiana benthamiana; NCBI TaxID: 4100; scientific name: Zea mays (Maize); NCBI TaxID: 4577;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-01 02:06:01	ID requested
⏵ 1	2023-11-09 05:57:13	announced

Dataset with its publication pending

submitter keyword: EAR motifs, effector protein, AP2/ERF transcription factors,TOPLESS, Ustilago maydis

HirofumiNakagami
contact affiliation	Basic Immune System of Plants / Protein Mass Spectrometry Max Planck Institute for Plant Breeding Research Carl-von-Linne-Weg 10, 50829 Cologne, Germany
contact email	nakagami@mpipz.mpg.de
lab head
Sara ChristinaStolze
contact affiliation	Plant Proteomics and Mass Spectrometry Group Max Planck Institute for Plant Breeding Research Carl-von-Linné Weg 10 50829 Cologne
contact email	stolze@mpipz.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD042605

PRIDE project URI

• PRIDE
  1. PXD042605
     1. Label: PRIDE project
     2. Name: The fungal pathogen Ustilago maydis targets the maize corepressor TPL2 to modulate host transcription for tumorigenisis