PXD042584

PXD042584 is an original dataset announced via ProteomeXchange.

Title	CXMS BS3 Ras/BRAF/MEK/1433 complex
Description	RAF-family kinases are activated by recruitment to the plasma membrane by GTP-bound RAS, where they initiate signaling through the MAP kinase cascade. Here we crosslinked (BS3), digested (trypsin), and analyzed via LC-MS/MS KRAS (human, residues 1-169) bound to intact BRAF (human) in an autoinhibited state with MEK1 (human, ) and a 14-3-3 (SF9) dimer . Analysis of this KRAS/BRAF/MEK1/14-3-3 complex reveals both interprotein and intraprotein crosslinks.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:10:41.446.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anna Schmoker
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Spodoptera frugiperda; NCBI TaxID: 7108;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-31 09:41:18	ID requested
1	2023-10-24 12:59:58	announced
⏵ 2	2023-11-14 09:10:44	announced	2023-11-14: Updated project metadata.

Park E, Rawson S, Schmoker A, Kim BW, Oh S, Song K, Jeon H, Eck MJ, Cryo-EM structure of a RAS/RAF recruitment complex. Nat Commun, 14(1):4580(2023) [pubmed]

submitter keyword: CXMS, human, Braf, Mek1, 14-3-3, SF9, Kras

Michael Eck
contact affiliation	Biological Chemistry and Molecular Pharmacology, Harvard Medical School Cancer Biology, Dana-Farber Cancer Institute
contact email	eck@crystal.harvard.edu
lab head
Anna Schmoker
contact affiliation	Dana-Farber Cancer Institute, Harvard Medical School, The Broad Institute of MIT and Harvard
contact email	aschmoker@crystal.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD042584

PRIDE project URI

• PRIDE
  1. PXD042584
     1. Label: PRIDE project
     2. Name: CXMS BS3 Ras/BRAF/MEK/1433 complex