PXD042577

PXD042577 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Trazodone is neuroprotective via rescue of dysregulated synaptic and mitochondrial translatomes in neurodegeneration
Description	We aime to discover changes which occur at the hippocampal nascent proteome prion to the neurodegeneration characteristic of prion disease and how this prion signature difer from that of proven neuroprotective drug trazodone hydrochloride. To label the nacent proteome we used non-canonical aminoacid tagging (NCAT) labelling all cell types within the hippocampus with L-azidohomoalanine (AHA) as well as azinorleucine (ANL) to label specifically Camk2a or GFAP neurons or astrocytes respectivly. We gave methionine surrogates mixed in drinking water and soggy mash for 1 week after which animals were sacrificed and both hippocampi extracted. Affinity purification of this samples showed a restoration of Mitochondrial and syaptic proteins decrease with prion disease which 2 week trazdoone treatement restored.
HostingRepository	PRIDE
AnnounceDate	2024-05-24
AnnouncementXML	Submission_2024-05-24_03:00:24.883.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hector Albert Gasco
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-31 07:43:38	ID requested
⏵ 1	2024-05-24 03:00:25	announced

Publication List

10.1093/brain/awad313;
Albert-Gasco H, Smith HL, Alvarez-Castelao B, Swinden D, Halliday M, Janaki-Raman S, Butcher AJ, Mallucci GR, Trazodone rescues dysregulated synaptic and mitochondrial nascent proteomes in prion neurodegeneration. Brain, 147(2):649-664(2024) [pubmed]

10.1093/brain/awad313;

Albert-Gasco H, Smith HL, Alvarez-Castelao B, Swinden D, Halliday M, Janaki-Raman S, Butcher AJ, Mallucci GR, Trazodone rescues dysregulated synaptic and mitochondrial nascent proteomes in prion neurodegeneration. Brain, 147(2):649-664(2024) [pubmed]

Keyword List

submitter keyword: synapse, UPR/ISR, trazodone,neurodegeneration, translational repression, mutochondria, translatome

submitter keyword: synapse, UPR/ISR, trazodone,neurodegeneration, translational repression, mutochondria, translatome

Contact List

Giovanna Mallucci
contact affiliation	Cambridge institute of Science, Altos Labs, Cambridge, UK
contact email	gmallucci@altoslabs.com
lab head
Hector Albert Gasco
contact affiliation	Altos labs
contact email	hector.hv2@gmail.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD042577
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD042577

PRIDE project URI

Repository Record List

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