⮝ Full datasets listing

PXD042573

PXD042573 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRapid and Easy Gel Disruption by Passing Through Pipette Tip with Centrifugation
DescriptionWe have developed an Easy Disruption method for Gel using pipet Tips (EDGe-Tip), in which polyacrylamide gel is passed through pipette tips by centrifugation in a few minutes to crush it into appropriately sized fragments. The size of the disrupted gel could be controlled by several parameters such as the strength of the centrifugal force and the size of the pipette tip hole. In shotgun proteomics, GeLC/MS/MS analysis combining SDS-PAGE and LC/MS/MS requires dicing and transferring gels to microtubes by hand, which are essential but annoying steps. Thus, EDGe-Tip was applied to the GeLC/MS/MS procedure instead of those steps. Peptides could be extracted more efficiently and variation in protein quantification is lower with EDGe-Tip than with manual dicing. We believe that this simple and rapid method of disrupting polyacrylamide gels can be useful for GeLC/MS/MS analysis and applied for experiments to extract biomolecules from the gels.
HostingRepositoryjPOST
AnnounceDate2024-05-31
AnnouncementXMLSubmission_2024-05-30_08:00:20.037.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHiroshi Nishida
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; Acetyl
Instrumentinstrument
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-05-31 05:34:19ID requested
12024-05-30 08:00:20announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: In-gel digestion, SDS-PAGE, Gel disruption, GeLC/MS/MS
Contact List
Yasushi Ishihama
lab head
Hiroshi Nishida
contact affiliationKyoto university
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002068/