PXD042573

PXD042573 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Rapid and Easy Gel Disruption by Passing Through Pipette Tip with Centrifugation
Description	We have developed an Easy Disruption method for Gel using pipet Tips (EDGe-Tip), in which polyacrylamide gel is passed through pipette tips by centrifugation in a few minutes to crush it into appropriately sized fragments. The size of the disrupted gel could be controlled by several parameters such as the strength of the centrifugal force and the size of the pipette tip hole. In shotgun proteomics, GeLC/MS/MS analysis combining SDS-PAGE and LC/MS/MS requires dicing and transferring gels to microtubes by hand, which are essential but annoying steps. Thus, EDGe-Tip was applied to the GeLC/MS/MS procedure instead of those steps. Peptides could be extracted more efficiently and variation in protein quantification is lower with EDGe-Tip than with manual dicing. We believe that this simple and rapid method of disrupting polyacrylamide gels can be useful for GeLC/MS/MS analysis and applied for experiments to extract biomolecules from the gels.
HostingRepository	jPOST
AnnounceDate	2024-05-31
AnnouncementXML	Submission_2024-05-30_08:00:20.037.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hiroshi Nishida
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; Acetyl
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-31 05:34:19	ID requested
⏵ 1	2024-05-30 08:00:20	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: In-gel digestion, SDS-PAGE, Gel disruption, GeLC/MS/MS

submitter keyword: In-gel digestion, SDS-PAGE, Gel disruption, GeLC/MS/MS

Contact List

Yasushi Ishihama
lab head
Hiroshi Nishida
contact affiliation	Kyoto university
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002068/