PXD042571

PXD042571 is an original dataset announced via ProteomeXchange.

Title	In situ ribosome clustering and ultrastructure reorganization of polar tube proteins in germinated microsporidia polar tube
Description	During host cell invasion, microsporidia translocate their entire cytoplasmic contents through a thin, hollow superstructure known as the polar tube. To achieve this, the polar tube transitions from a compact spring-like state inside the environmental spore to a long needle-like tube capable of longrange sporoplasm delivery. The unique mechanical properties of the building blocks of the polar tube allow for an explosive transition from compact to extended state and support the rapid cargo translocation process. The molecular and structural factors enabling this ultrafast process and the ultrastructural changes during cargo delivery remain a mystery. Here, we employ light microscopy and in situ cryo-electron tomography to visualize multiple ultrastructural states of the polar tube, allowing us to evaluate the kinetics of its germination and characterize the underlying morphological transitions. We describe a cargo-filled state with a unique ordered arrangement of microsporidian ribosomes, which cluster along the thin tube wall, and an empty post-translocation state with a reduced diameter but a thicker wall. Together with endogenous compositional information from an affinity-purified polar tube, our work provides comprehensive novel data on the infection apparatus of microsporidia and demonstrates that ribosomes are efficiently transported through polar tubes in a spiral-like parallel arrangement.
HostingRepository	PRIDE
AnnounceDate	2024-01-09
AnnouncementXML	Submission_2024-01-08_23:12:28.816.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Johannes Fuchs
SpeciesList	scientific name: Vairimorpha necatrix; NCBI TaxID: 6039;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-31 02:54:33	ID requested
⏵ 1	2024-01-08 23:12:29	announced

Dataset with its publication pending

submitter keyword: Ribosome, Polar tube, Polar tube proteins,Microsporidia, Cryo-Electron tomography

Himanshu Sharma
contact affiliation	Department of Medical Biochemistry and Biophysics, University of Umea, Sweden
contact email	himanshu.sharma@umu.se
lab head
Johannes Fuchs
contact affiliation	Gothenburg University
contact email	johannes.fuchs@gu.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042571
     1. Label: PRIDE project
     2. Name: In situ ribosome clustering and ultrastructure reorganization of polar tube proteins in germinated microsporidia polar tube